Allelic variants of the Plasmodium falciparum merozoite surface antigen 2 (MSA-2) in a geographically restricted area of Irian Jaya

Vikki M. Marshall, Ronald L. Anthony, Michael J. Bangs, Purnomo, Robin F. Anders, Ross L. Coppel

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Blood samples were collected from 12 residents of 4 villages in the Oksibil area of Irian Jaya. Eleven patients were positive for Plasmodium falciparum infection as evidenced by successful amplification of the MSA-2 gene by the polymerase chain reaction. Two patients showed evidence of infection by 2 strains of Plasmodium falciparum. All MSA2 genes were completely sequenced and all could be assigned to one of the two major allelic families of MSA-2, however all MSA-2 gene sequences differed from previously described alleles. Five new allelic forms were identified, one of which was present in 8 of the 11 patients. Within small natural populations of P. falciparum, it appears that variation in MSA-2 approximates that seen world-wide. All samples were also analysed by hybridisation of amplified DNA to family specific probes and all samples hybridised to known probes. Our results demonstrate that there is a degree of microheterogeneity of MSA-2 that is undetectable by hybridisation studies alone.

Original languageEnglish
Pages (from-to)13-21
Number of pages9
JournalMolecular and Biochemical Parasitology
Issue number1
Publication statusPublished - 1 Jan 1994
Externally publishedYes


  • Allelic variant
  • Irian Jaya
  • Merozoite surface antigen 2 (MSA-2)
  • Nucleotide sequencing
  • Oligonucleotide probe
  • Plasmodium falciparum

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