Allele-specific expression of imprinted genes in mouse migratory primordial germ cells

Piroska E. Szabó, Karin Hübner, Hans Schöler, Jeffrey R. Mann

Research output: Contribution to journalArticleResearchpeer-review

250 Citations (Scopus)

Abstract

In somatic cells, imprinted genes are expressed monoallelically according to parent-of-origin. In contrast, in 11.5 days post-coitum primordial germ cells (PGCs), and later stage germ cells, these same genes are expressed biallelically, suggesting that imprints inherited from the gametes are largely erased by this stage. To determine when in germ cell development this biallelic expression phenomenon commences, we isolated migrating PGCs by flow cytometry and determined the allele-specific expression of four imprinted genes - Snrpn, Igf2, H19 and Igf2r. The first three genes were expressed monoallelically, while the latter gene was expressed biallelically. These results show that inherited imprints regulating monoallelic expression are largely intact in migrating PGCs.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalMechanisms of Development
Volume115
Issue number1-2
DOIs
Publication statusPublished - 20 Jun 2002
Externally publishedYes

Keywords

  • Epigenetics
  • Flow cytometry
  • Gene expression
  • Germ cell
  • Germ line
  • Green fluorescent protein
  • Imprinting
  • Primordial germ cell
  • Reverse transcription-polymerase chain reaction
  • Transgene

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