The activation of the Akt signalling in response to cytokine receptor signalling promotes protein synthesis, cellular growth and proliferation. To determine the role of Akt in interleukin-3 (IL-3) signalling, we generated IL-3-dependent myeloid cell lines from mice lacking Akt1, Akt2 or Akt3. Akt1 deletion resulted in accelerated apoptosis at low concentrations of IL-3. Expression of constitutively active Akt1 was sufficient to delay apoptosis in response to IL-3 withdrawal, but not sufficient to induce proliferation in the absence of IL-3. Akt1 prolonged survival of Bim- or Bad-deficient cells, but not cells lacking Puma, indicating that Akt1-dependent repression of apoptosis was in part dependent on Puma and independent of Bim or Bad. Our data show that a key role of Akt1 during IL-3 signalling is to repress p53-dependent apoptosis pathways, including transcriptional upregulation of Puma. Moreover, our data indicate that regulation of BH3-only proteins by Akt is dispensable for Akt-dependent cell survival. ? 2013 Macmillan Publishers Limited All rights reserved.
Green, B. D., Jabbour, A. M.
, Sandow, J. J., Riffkin, C. D., Masouras, D., Daunt, C. P., ... Ekert, P. G. (2013). Akt1 is the principal Akt isoform regulating apoptosis in limiting cytokine concentrations
. Cell Death and Differentiation
(10), 1341 - 1349. https://doi.org/10.1038/cdd.2013.63