Akt1 is the principal Akt isoform regulating apoptosis in limiting cytokine concentrations

Benjamin D Green, Anissa M Jabbour, Jarrod J Sandow, Christopher D Riffkin, D Masouras, Carmel Patricia Daunt, Marika Salmanidis, Gabriela Brumatti, Brian Hemmings, Mark Andrew Guthridge, Richard Bruce Pearson, Paul G Ekert

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26 Citations (Scopus)


The activation of the Akt signalling in response to cytokine receptor signalling promotes protein synthesis, cellular growth and proliferation. To determine the role of Akt in interleukin-3 (IL-3) signalling, we generated IL-3-dependent myeloid cell lines from mice lacking Akt1, Akt2 or Akt3. Akt1 deletion resulted in accelerated apoptosis at low concentrations of IL-3. Expression of constitutively active Akt1 was sufficient to delay apoptosis in response to IL-3 withdrawal, but not sufficient to induce proliferation in the absence of IL-3. Akt1 prolonged survival of Bim- or Bad-deficient cells, but not cells lacking Puma, indicating that Akt1-dependent repression of apoptosis was in part dependent on Puma and independent of Bim or Bad. Our data show that a key role of Akt1 during IL-3 signalling is to repress p53-dependent apoptosis pathways, including transcriptional upregulation of Puma. Moreover, our data indicate that regulation of BH3-only proteins by Akt is dispensable for Akt-dependent cell survival. ? 2013 Macmillan Publishers Limited All rights reserved.
Original languageEnglish
Pages (from-to)1341 - 1349
Number of pages9
JournalCell Death and Differentiation
Issue number10
Publication statusPublished - 2013

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