Aggregation of the naturally occurring lipopeptide, surfactin, at interfaces and in solution: an unusual type of surfactant?

Hsin-Hui Shen, Robert Thomas, Chien-Yen Chen, Richard Darton, Simon Baker, Jeffrey Penfold

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64 Citations (Scopus)

Abstract

Neutron reflectometry has been used to study the structure of the biosurfactant, surfactin, at the air/water and at the hydrophobic solid/water interfaces. Three different deuterated surfactins were produced from the Bacillus subtilis strain: one perdeuterated, one with the four leucines perdeuterated, and one with everything except the four leucines perdeuterated. The neutron reflectivity profiles of these three samples in null reflecting water and in D20 with a seventh profile of the protonated surfactin in D2O were measured at pH 7.5. This combination of different isotopic compositions made it possible to deduce the distribution of each type of labeled fragment in the surfactin. Surfactin is found to adopt a ball-like structure with a thickness of 14 +/- A and an area per molecule of 147 +/- 5 A2. This makes it more like a hydrophobic nanoparticle, whose solubility in water is maintained only by its charge, than a conventional surfactant. This is probably what makes it surface-active at such low concentrations and what contributes to its forming very compact surface layers that are more dense than observed for most conventional amphiphiles. The reflectivity data were fitted by a model in which the structure of surfactin was divided into three fragments: the four leucines taken as a group, the hydrocarbon chain, and a hydrophilic group containing the two negative charges. An analysis of the reflectivity gave the following separations between fragments, where zero corresponds to the Gibbs plane for zero water adsorption:...
Original languageEnglish
Pages (from-to)4211 - 4218
Number of pages8
JournalLangmuir
Volume25
Issue number7
DOIs
Publication statusPublished - 2009
Externally publishedYes

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