Aggregation of embryos and embryonic stem cells

Ronald Maatman, Marina Gertsenstein, Emile de Meijer, Andras Nagy, Kristina Vintersten

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

3 Citations (Scopus)

Abstract

The human genome has been almost completely sequenced, and at least 30,000 genes have been found (1). Systematic studies of gene expression patterns by using cDNA microarrays have provided a powerful approach to molecular dissection of cells and tissues by comparing expression levels of tens of thousands of these genes at a time. Even insight into signaling pathways has been gained (2,3). However, information about the in vivo function of the various genes, especially disease genes, still requires the development of animal models carrying particular mutations. Several mouse mutagenesis projects (see Chapter 13) have been started during the last decade, and the number of mutant mice generated by targeted mutagenesis in mouse embryonic stem (ES) cells has increased exponentially (4–7).
Original languageEnglish
Title of host publicationTransgenic Mouse
Subtitle of host publicationMethods and Protocols
EditorsMarten H. Hofker, Jan Deursen
Place of PublicationTotowa, NJ USA
PublisherHumana Press
Chapter11
Pages201-230
Number of pages30
Volume209
Edition1st
ISBN (Electronic)9781592593408
DOIs
Publication statusPublished - 2003
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume209
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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