Aggregation chimeras: Combining es cells, diploid, and tetraploid embryos

Mika Tanaka, Anna Katerina Hadjantonakis, Kristina Vintersten, Andras Nagy

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

18 Citations (Scopus)

Abstract

During the past 40 years, mouse chimeras have served as invaluable tools for studying not only genetics but also embryonic development, and the path from undifferentiated cell populations to fully committed functional cell types. This chapter gives a description of the early events of cell commitment and differentiation in the pre-and postimplantation-stage embryo. Next, a discussion follows highlighting the most commonly used as well as more recently developed applications of various cell types and origins used in the production of chimeras. Finally, detailed protocols and trouble-shooting suggestions will be presented for each of the steps involved.

Original languageEnglish
Title of host publicationGene Knockout Protocols
Subtitle of host publicationSecond Edition
Pages287-309
Number of pages23
Volume530
DOIs
Publication statusPublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume530
ISSN (Print)1064-3745

Keywords

  • aggregation
  • chimera
  • embryo
  • ES cells
  • mouse
  • tetraploid

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