TY - JOUR
T1 - Age-related decline in stress responses of human myocardium may not be explained by changes in mtDNA
AU - Miller, Francis J
AU - Nagley, Phillip
AU - Mariani, Justin
AU - Ou, Ruchong
AU - Liu, Vincent W S
AU - Zhang, Chunfang
AU - Linnane, Anthony W
AU - Pepe, Salvatore
AU - Rosenfeldt, Franklin Lawrence
PY - 2009
Y1 - 2009
N2 - Elderly patients undergoing cardiac surgery are more likely to suffer postoperative heart failure than younger patients. This phenomenon is mirrored by an age-related loss of mitochondrial function and by an in vitro loss of myocardial contractile force following a stress. To examine the possibility that loss of mtDNA integrity may be responsible, we quantified representative age-associated mtDNA mutations (mtDNA4977 and mtDNAA3243G) and mtDNA copy number using quantitative polymerase chain reaction in atrial samples obtained during cardiac surgery. The myocardium underwent organ bath contractility testing before and after either an ischaemic or hypoxic stress. We found that with age, recovery of developed force after either stressor significantly declined (P
AB - Elderly patients undergoing cardiac surgery are more likely to suffer postoperative heart failure than younger patients. This phenomenon is mirrored by an age-related loss of mitochondrial function and by an in vitro loss of myocardial contractile force following a stress. To examine the possibility that loss of mtDNA integrity may be responsible, we quantified representative age-associated mtDNA mutations (mtDNA4977 and mtDNAA3243G) and mtDNA copy number using quantitative polymerase chain reaction in atrial samples obtained during cardiac surgery. The myocardium underwent organ bath contractility testing before and after either an ischaemic or hypoxic stress. We found that with age, recovery of developed force after either stressor significantly declined (P
UR - http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%234933%239999%23999999999%2399999%23FLA%23&_cdi=4933&_pubType=J&_auth=y&_acct=C
U2 - 10.1016/j.mad.2009.09.003
DO - 10.1016/j.mad.2009.09.003
M3 - Article
VL - 130
SP - 742
EP - 747
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 11-12
ER -