Age-related decline in stress responses of human myocardium may not be explained by changes in mtDNA

Francis J Miller; Phillip Nagley; Justin Mariani; Ruchong Ou; Vincent W S Liu; Chunfang Zhang; Anthony W Linnane; Salvatore Pepe; Franklin Lawrence Rosenfeldt

doi:10.1016/j.mad.2009.09.003

Age-related decline in stress responses of human myocardium may not be explained by changes in mtDNA

Francis J Miller, Phillip Nagley, Justin Mariani, Ruchong Ou, Vincent W S Liu, Chunfang Zhang, Anthony W Linnane, Salvatore Pepe, Franklin Lawrence Rosenfeldt

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

Elderly patients undergoing cardiac surgery are more likely to suffer postoperative heart failure than younger patients. This phenomenon is mirrored by an age-related loss of mitochondrial function and by an in vitro loss of myocardial contractile force following a stress. To examine the possibility that loss of mtDNA integrity may be responsible, we quantified representative age-associated mtDNA mutations (mtDNA4977 and mtDNAA3243G) and mtDNA copy number using quantitative polymerase chain reaction in atrial samples obtained during cardiac surgery. The myocardium underwent organ bath contractility testing before and after either an ischaemic or hypoxic stress. We found that with age, recovery of developed force after either stressor significantly declined (P

Original language	English
Pages (from-to)	742 - 747
Number of pages	6
Journal	Mechanisms of Ageing and Development
Volume	130
Issue number	11-12
DOIs	https://doi.org/10.1016/j.mad.2009.09.003
Publication status	Published - 2009

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title = "Age-related decline in stress responses of human myocardium may not be explained by changes in mtDNA",

abstract = "Elderly patients undergoing cardiac surgery are more likely to suffer postoperative heart failure than younger patients. This phenomenon is mirrored by an age-related loss of mitochondrial function and by an in vitro loss of myocardial contractile force following a stress. To examine the possibility that loss of mtDNA integrity may be responsible, we quantified representative age-associated mtDNA mutations (mtDNA4977 and mtDNAA3243G) and mtDNA copy number using quantitative polymerase chain reaction in atrial samples obtained during cardiac surgery. The myocardium underwent organ bath contractility testing before and after either an ischaemic or hypoxic stress. We found that with age, recovery of developed force after either stressor significantly declined (P",

author = "Miller, {Francis J} and Phillip Nagley and Justin Mariani and Ruchong Ou and Liu, {Vincent W S} and Chunfang Zhang and Linnane, {Anthony W} and Salvatore Pepe and Rosenfeldt, {Franklin Lawrence}",

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issn = "0047-6374",

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}

Age-related decline in stress responses of human myocardium may not be explained by changes in mtDNA. / Miller, Francis J; Nagley, Phillip ; Mariani, Justin ; Ou, Ruchong; Liu, Vincent W S; Zhang, Chunfang; Linnane, Anthony W; Pepe, Salvatore; Rosenfeldt, Franklin Lawrence.

In: Mechanisms of Ageing and Development, Vol. 130, No. 11-12, 2009, p. 742 - 747.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - Age-related decline in stress responses of human myocardium may not be explained by changes in mtDNA

AU - Miller, Francis J

AU - Nagley, Phillip

AU - Mariani, Justin

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AU - Liu, Vincent W S

AU - Zhang, Chunfang

AU - Linnane, Anthony W

AU - Pepe, Salvatore

AU - Rosenfeldt, Franklin Lawrence

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AB - Elderly patients undergoing cardiac surgery are more likely to suffer postoperative heart failure than younger patients. This phenomenon is mirrored by an age-related loss of mitochondrial function and by an in vitro loss of myocardial contractile force following a stress. To examine the possibility that loss of mtDNA integrity may be responsible, we quantified representative age-associated mtDNA mutations (mtDNA4977 and mtDNAA3243G) and mtDNA copy number using quantitative polymerase chain reaction in atrial samples obtained during cardiac surgery. The myocardium underwent organ bath contractility testing before and after either an ischaemic or hypoxic stress. We found that with age, recovery of developed force after either stressor significantly declined (P

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