Age-related decline in stress responses of human myocardium may not be explained by changes in mtDNA

Francis J Miller, Phillip Nagley, Justin Mariani, Ruchong Ou, Vincent W S Liu, Chunfang Zhang, Anthony W Linnane, Salvatore Pepe, Franklin Lawrence Rosenfeldt

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Abstract

Elderly patients undergoing cardiac surgery are more likely to suffer postoperative heart failure than younger patients. This phenomenon is mirrored by an age-related loss of mitochondrial function and by an in vitro loss of myocardial contractile force following a stress. To examine the possibility that loss of mtDNA integrity may be responsible, we quantified representative age-associated mtDNA mutations (mtDNA4977 and mtDNAA3243G) and mtDNA copy number using quantitative polymerase chain reaction in atrial samples obtained during cardiac surgery. The myocardium underwent organ bath contractility testing before and after either an ischaemic or hypoxic stress. We found that with age, recovery of developed force after either stressor significantly declined (P
Original languageEnglish
Pages (from-to)742 - 747
Number of pages6
JournalMechanisms of Ageing and Development
Volume130
Issue number11-12
DOIs
Publication statusPublished - 2009

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