Age-related changes of P2X1 receptor mRNA in the bladder detrusor from men with and without bladder outlet obstruction

Wendy Chen-Nee Chua, Lu Liu, Kylie J Mansfield, Kenneth J Vaux, Kate H Moore, Richard J Millard, Elizabeth Burcher

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20 Citations (Scopus)


The urinary bladder purinergic system is reported to change with age and with bladder dysfunction. Here, we examined the expression of purinergic P2X(1) receptors in detrusor and mucosa (urothelium+lamina propria) from male control bladder and investigated age-related P2X(1) receptor mRNA expression in control and obstructed detrusor. Biopsy specimens were obtained at cystoscopy from control patients (n=46, age range 30-86years) and patients diagnosed with outlet obstruction (n=29, 46-88years). Calponin expression (measured by RT-PCR) was similar in control and obstructed detrusor and did not change with age. Quantitative competitive RT-PCR was used to measure P2X(1) receptor and GAPDH mRNA in control and obstructed detrusor. P2X(1) receptor mRNA expression was 9-fold (p
Original languageEnglish
Pages (from-to)686 - 692
Number of pages7
JournalExperimental Gerontology
Issue number7
Publication statusPublished - 2007
Externally publishedYes

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