Age dictates a steroid-resistant cascade of Wnt5a, transglutaminase 2, and leukotrienes in inflamed airways

Katharina Dietz, Marta de los Reyes Jiménez, Eva S. Gollwitzer, Adam M. Chaker, Ulrich M. Zissler, Olof P. Rådmark, Hoeke A. Baarsma, Melanie Königshoff, Carsten B. Schmidt-Weber, Benjamin J. Marsland, Julia Esser-von Bieren

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Background Airway remodeling is a detrimental and refractory process showing age-dependent clinical manifestations that are mechanistically undefined. The leukotriene (LT) and wingless/integrase (Wnt) pathways have been implicated in remodeling, but age-specific expression profiles and common regulators remained elusive. Objective We sought to study the activation of the LT and Wnt pathways during early- or late-onset allergic airway inflammation and to address regulatory mechanisms and clinical relevance in normal human bronchial epithelial cells (NHBEs) and nasal polyp tissues. Methods Mice were sensitized with house dust mite (HDM) allergens from days 3, 15, or 60 after birth. Remodeling factors in murine bronchoalveolar lavage fluid, lung tissue, or human nasal polyp tissue were analyzed by means of Western blotting, immunoassays, or histology. Regulatory mechanisms were studied in cytokine/HDM-stimulated NHBEs and macrophages. Results Bronchoalveolar lavage fluid LT levels were increased in neonatal and adult but reduced in juvenile HDM-sensitized mice. Lungs of neonatally sensitized mice showed increased 5-lipoxygenase levels, whereas adult mice expressed more group 10 secretory phospholipase A2, Wnt5a, and transglutaminase 2 (Tgm2). Older mice showed colocalization of Wnt5a and LT enzymes in the epithelium, a pattern also observed in human nasal polyps. IL-4 promoted epithelial Wnt5a secretion, which upregulated macrophage Tgm2 expression, and Tgm2 inhibition in turn reduced LT release. Tgm2, group 10 secretory phospholipase A2, and LT enzymes in NHBEs and nasal polyps were refractory to corticosteroids. Conclusion Our findings reveal age differences in LT and Wnt pathways during airway inflammation and identify a steroid-resistant cascade of Wnt5a, Tgm2, and LTs, which might represent a therapeutic target for airway inflammation and remodeling.

Original languageEnglish
Pages (from-to)1343-1354.e6
Number of pages18
JournalJournal of Allergy and Clinical Immunology
Issue number4
Publication statusPublished - 1 Apr 2017
Externally publishedYes


  • Age
  • airway inflammation
  • airway remodeling
  • allergy
  • asthma
  • bronchial epithelial cells
  • house dust mite
  • leukotrienes
  • lipid mediators
  • nasal polyps
  • secretory phospholipase A
  • transglutaminase 2
  • Wnt5a

Cite this

Dietz, K., de los Reyes Jiménez, M., Gollwitzer, E. S., Chaker, A. M., Zissler, U. M., Rådmark, O. P., Baarsma, H. A., Königshoff, M., Schmidt-Weber, C. B., Marsland, B. J., & Esser-von Bieren, J. (2017). Age dictates a steroid-resistant cascade of Wnt5a, transglutaminase 2, and leukotrienes in inflamed airways. Journal of Allergy and Clinical Immunology, 139(4), 1343-1354.e6.