Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood

Elena Blanco, Martín Pérez-Andrés, Sonia Arriba-Méndez, Teresa Contreras-Sanfeliciano, Ignacio Criado, Ondrej Pelak, Ana Serra-Caetano, Alfonso Romero, Noemí Puig, Ana Remesal, Juan Torres Canizales, Eduardo López-Granados, Tomas Kalina, Ana E. Sousa, Menno van Zelm, Mirjam van der Burg, Jacques J.M. van Dongen, Alberto Orfao, on behalf of the EuroFlow PID group

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161 Citations (Scopus)


Background: Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility to infection, immunodeficiency, autoimmunity, and neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH isotype distribution within memory B-cell (MBC) and plasma cell (PCs) compartments remains to be investigated. Objective: We studied the age-related distribution of MBCs and PCs expressing different IgH isotypes in addition to the immature/transitional and naive B-cell compartments. Methods: B-cell and PC subsets and plasma IgH isotype levels were studied in cord blood (n = 19) and peripheral blood (n = 215) from healthy donors aged 0 to 90 years by using flow cytometry and nephelometry, respectively. Results: IgH-switched MBCs expressing IgG 1 , IgG 2 , IgG 3 , IgA 1 , and IgA 2 were already detected in cord blood and newborns at very low counts, whereas CD27 + IgM ++ IgD + MBCs only became detectable at 1 to 5 months and remained stable until 2 to 4 years, and IgD MBCs peaked at 2 to 4 years, with both populations decreasing thereafter. MBCs expressing IgH isotypes of the second immunoglobulin heavy chain constant region (IGHC) gene block (IgG 1 , IgG 3 , and IgA 1 ) peaked later during childhood (2-4 years), whereas MBCs expressing third IGHC gene block immunoglobulin isotypes (IgG 2 , IgG 4 , and IgA 2 ) reached maximum values during adulthood. PCs were already detected in newborns, increasing in number until 6 to 11 months for IgM, IgG 1 , IgG 2 , IgG 3 , IgA 1 , and IgA 2 ; until 2 to 4 years for IgD; and until 5 to 9 years for IgG 4 and decreasing thereafter. For most IgH isotypes (except IgD and IgG 4 ), maximum plasma levels were reached after PC and MBC counts peaked. Conclusions: PC counts reach maximum values early in life, followed by MBC counts and plasma IgH isotypes. Importantly, IgH isotypes from different IGHC gene blocks show different patterns, probably reflecting consecutive cycles of IgH isotype switch recombination through life.

Original languageEnglish
Pages (from-to)2208-2219.e16
Number of pages28
JournalThe Journal of Allergy and Clinical Immunology
Issue number6
Publication statusPublished - 1 Jun 2018


  • age-related values
  • flow cytometry
  • IgH isotype
  • Immunoglobulins
  • memory B cells
  • normal B cells
  • plasma cells
  • reference ranges
  • subclass

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