Affinofile profiling: How efficiency of CD4/CCR5 usage impacts the biological and pathogenic phenotype of HIV

Kelechi Chikere, Tom Chou, Paul R. Gorry, Benhur Lee

Research output: Contribution to journalReview ArticleResearchpeer-review

19 Citations (Scopus)

Abstract

HIV-1 envelope (Env) uses CD4 and a coreceptor (CCR5 and/or CXCR4) for viral entry. The efficiency of receptor/coreceptor mediated entry has important implications for HIV pathogenesis and transmission. The advent of CCR5 inhibitors in clinical use also underscores the need for quantitative and predictive tools that can guide therapeutic management. Historically, measuring the efficiency of CD4/CCR5 mediated HIV entry has relied on surrogate and relatively slow throughput assays that cannot adequately capture the full spectrum of Env phenotypes. In this review, we discuss the details of the Affinofile receptor affinity profiling system that has provided a quantitative and higher throughput method to characterize viral entry efficiency as a function of CD4 and CCR5 expression levels. We will then review how the Affinofile system has been used to reveal the distinct pathophysiological properties associated with Env entry phenotypes and discuss potential shortcomings of the current system.

Original languageEnglish
Pages (from-to)81-91
Number of pages11
JournalVirology
Volume435
Issue number1
DOIs
Publication statusPublished - 5 Jan 2013

Keywords

  • CCR5
  • CD4
  • Cellular tropism
  • Entry efficiency
  • Entry inhibitors
  • Env
  • HIV-1
  • Pathogenesis

Cite this