Abstract
Formation of a macromolecular complex between the RNA binding protein HIV-1 Rev and HIV-1 mRNA is an essential prerequisite for nuclear export and subsequent expression of HIV-1 mRNA. The arginine rich peptide TRQARRNRRRRWRARQR, corresponding to residues 34-50 of HIV-1 Rev, contains the mRNA binding motif. We prepared a thioether linked Rev34-50-cellulose conjugate to affinity purify a fragment of synthetic mRNA corresponding to the high affinity binding site for Rev. The correctly folded fraction of mRNA (27.5%) was isolated from a crude synthetic mixture.
Original language | English |
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Pages (from-to) | 47-50 |
Number of pages | 4 |
Journal | Biomedical Peptides, Proteins & Nucleic Acids: Structure, Synthesis & Biological Activity |
Volume | 2 |
Issue number | 2 |
Publication status | Published - 1 Jan 1996 |
Externally published | Yes |