Affinity-purification and identification of GrpE homologues from mammalian mitochondria

Dean J. Naylor, Michael T. Ryan, Rosemary Condron, Nicholas J. Hoogenraad, Peter B. Høj

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We used affinity chromatography on DnaK columns to identify a mitochondrial GrpE homologue from bovine, porcine and rat liver mitochondria. The 24 kDa GrpE homologue bound specifically to the DnaK column and was not eluted with 1 M KCl but readily with 5 mM ATP. Sequence analysis of the bovine homologue (85 residues) revealed 42% positional identity to mitochondrial GrpEp from S. cerevisiae and about 30% identity to the bacterial counterparts. Thus, GrpE homologues from higher and lower eukaryotes are highly conserved.

Original languageEnglish
Pages (from-to)75-79
Number of pages5
JournalBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
Issue number1
Publication statusPublished - 5 Apr 1995
Externally publishedYes


  • Chaperone
  • Protein sequencing

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