Four independent methods have established that the structure of a previously reported pteridine affinity adsorbent, 6,7‐dimethyl‐5,6,7,8‐tetrahydropterin–CH‐Sepharose, is 5(CH‐Sepharosyl)‐6,7‐dimethyl‐5,6,7,8‐tetrahydropterin. A novel reaction, the carbodiimide‐promoted coupling of a carboxyl group to N‐5 of a tetrahydropterin, is described. Two novel adsorbents, 5‐formyl‐tetrahydrofolate–AH‐Sepharose and 5‐methyl‐tetrahydrofolate–AH‐Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase but also in the study of folate‐metabolizing enzymes.
|Number of pages||7|
|Journal||European Journal of Biochemistry|
|Publication status||Published - 1 Jan 1978|