Affinity Chromatography of Phenylalanine Hydroxylase: The Structure of a Pteridine Adsorbent

Richard G H Cotton, Ian G. Jennings

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Four independent methods have established that the structure of a previously reported pteridine affinity adsorbent, 6,7‐dimethyl‐5,6,7,8‐tetrahydropterin–CH‐Sepharose, is 5(CH‐Sepharosyl)‐6,7‐dimethyl‐5,6,7,8‐tetrahydropterin. A novel reaction, the carbodiimide‐promoted coupling of a carboxyl group to N‐5 of a tetrahydropterin, is described. Two novel adsorbents, 5‐formyl‐tetrahydrofolate–AH‐Sepharose and 5‐methyl‐tetrahydrofolate–AH‐Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase but also in the study of folate‐metabolizing enzymes.

Original languageEnglish
Pages (from-to)357-363
Number of pages7
JournalEuropean Journal of Biochemistry
Issue number2
Publication statusPublished - 1 Jan 1978
Externally publishedYes

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