TY - JOUR
T1 - Affinity Chromatography of Phenylalanine Hydroxylase
T2 - The Structure of a Pteridine Adsorbent
AU - Cotton, Richard G H
AU - Jennings, Ian G.
PY - 1978/1/1
Y1 - 1978/1/1
N2 - Four independent methods have established that the structure of a previously reported pteridine affinity adsorbent, 6,7‐dimethyl‐5,6,7,8‐tetrahydropterin–CH‐Sepharose, is 5(CH‐Sepharosyl)‐6,7‐dimethyl‐5,6,7,8‐tetrahydropterin. A novel reaction, the carbodiimide‐promoted coupling of a carboxyl group to N‐5 of a tetrahydropterin, is described. Two novel adsorbents, 5‐formyl‐tetrahydrofolate–AH‐Sepharose and 5‐methyl‐tetrahydrofolate–AH‐Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase but also in the study of folate‐metabolizing enzymes.
AB - Four independent methods have established that the structure of a previously reported pteridine affinity adsorbent, 6,7‐dimethyl‐5,6,7,8‐tetrahydropterin–CH‐Sepharose, is 5(CH‐Sepharosyl)‐6,7‐dimethyl‐5,6,7,8‐tetrahydropterin. A novel reaction, the carbodiimide‐promoted coupling of a carboxyl group to N‐5 of a tetrahydropterin, is described. Two novel adsorbents, 5‐formyl‐tetrahydrofolate–AH‐Sepharose and 5‐methyl‐tetrahydrofolate–AH‐Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase but also in the study of folate‐metabolizing enzymes.
UR - http://www.scopus.com/inward/record.url?scp=0017858587&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1978.tb12247.x
DO - 10.1111/j.1432-1033.1978.tb12247.x
M3 - Article
C2 - 648526
AN - SCOPUS:0017858587
VL - 85
SP - 357
EP - 363
JO - The FEBS Journal
JF - The FEBS Journal
SN - 1742-464X
IS - 2
ER -