Aerolysin and pertussis toxin share a common receptor-binding domain

Jamie Rossjohn, J. Thomas Buckley, Bart Hazes, Alexey G. Murzin, Randy J. Read, Michael W. Parker

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)

Abstract

We have discovered that the bacterial toxins aerolysin and pertussis toxin share a common domain. This is surprising because the two toxins affect cells in very different ways. The common domain, which we call the APT domain, consists of two three-stranded anti-parallel β-sheets that come together and wrap around a central pair of helices. The APT domain shares a common fold with the C-type lectins and Link modules, and there appears to be a divergent relationship among the three families. One surface region of the APT domain is highly conserved, raising the possibility that the domains have a common function in both proteins. Mutation of one of the conserved surface residues in aerolysin, Tyr61, results in reduced receptor binding and activity, thus providing evidence that the APT domain may be involved in interaction with the toxin's receptor. Structural and biochemical evidence suggests that the APT domain contains a carbohydrate-binding site that can direct the toxins to their target cells.

Original languageEnglish
Pages (from-to)3426-3434
Number of pages9
JournalThe EMBO Journal
Volume16
Issue number12
DOIs
Publication statusPublished - 16 Jun 1997
Externally publishedYes

Keywords

  • Aerolysin
  • C-type lectins
  • Carbohydrate binding
  • Pertussis toxin
  • Toxins

Cite this

Rossjohn, J., Buckley, J. T., Hazes, B., Murzin, A. G., Read, R. J., & Parker, M. W. (1997). Aerolysin and pertussis toxin share a common receptor-binding domain. The EMBO Journal, 16(12), 3426-3434. https://doi.org/10.1093/emboj/16.12.3426