Adult Neural Stem Cells from the Subventricular Zone Give Rise to Reactive Astrocytes in the Cortex after Stroke

Maryam Faiz, Nadia Sachewsky, Sergio Gascón, K. W.Annie Bang, Cindi M. Morshead, Andras Nagy

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Reactive astrocytes (RAs) have been reported to convert to multipotent neural stem cells (NSCs) capable of neurosphere (NS) formation and multilineage differentiation in vitro. Using genetic tagging, we determined that subventricular zone (SVZ) NSCs give rise to NSs derived from the stroke-injured cortex. We demonstrate that these cells can be isolated from the cortex in two different models of stroke and from different stroke-lesioned cortical regions. Interestingly, SVZ NSCs give rise to a subpopulation of RAs in the cortex that contribute to astrogliosis and scar formation. Last, we show that these SVZ derived RAs can be converted to neurons in vivo by forced expression of Ascl1. Identifying the contribution of cells originating from the SVZ to injury repair has implications for neural regeneration strategies.

Original languageEnglish
Pages (from-to)624-634
Number of pages11
JournalCell Stem Cell
Volume17
Issue number5
DOIs
Publication statusPublished - 5 Nov 2015
Externally publishedYes

Cite this

Faiz, Maryam ; Sachewsky, Nadia ; Gascón, Sergio ; Bang, K. W.Annie ; Morshead, Cindi M. ; Nagy, Andras. / Adult Neural Stem Cells from the Subventricular Zone Give Rise to Reactive Astrocytes in the Cortex after Stroke. In: Cell Stem Cell. 2015 ; Vol. 17, No. 5. pp. 624-634.
@article{6044e4bd3baa4ff291addce3c4e6bcde,
title = "Adult Neural Stem Cells from the Subventricular Zone Give Rise to Reactive Astrocytes in the Cortex after Stroke",
abstract = "Reactive astrocytes (RAs) have been reported to convert to multipotent neural stem cells (NSCs) capable of neurosphere (NS) formation and multilineage differentiation in vitro. Using genetic tagging, we determined that subventricular zone (SVZ) NSCs give rise to NSs derived from the stroke-injured cortex. We demonstrate that these cells can be isolated from the cortex in two different models of stroke and from different stroke-lesioned cortical regions. Interestingly, SVZ NSCs give rise to a subpopulation of RAs in the cortex that contribute to astrogliosis and scar formation. Last, we show that these SVZ derived RAs can be converted to neurons in vivo by forced expression of Ascl1. Identifying the contribution of cells originating from the SVZ to injury repair has implications for neural regeneration strategies.",
author = "Maryam Faiz and Nadia Sachewsky and Sergio Gasc{\'o}n and Bang, {K. W.Annie} and Morshead, {Cindi M.} and Andras Nagy",
year = "2015",
month = "11",
day = "5",
doi = "10.1016/j.stem.2015.08.002",
language = "English",
volume = "17",
pages = "624--634",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Elsevier",
number = "5",

}

Adult Neural Stem Cells from the Subventricular Zone Give Rise to Reactive Astrocytes in the Cortex after Stroke. / Faiz, Maryam; Sachewsky, Nadia; Gascón, Sergio; Bang, K. W.Annie; Morshead, Cindi M.; Nagy, Andras.

In: Cell Stem Cell, Vol. 17, No. 5, 05.11.2015, p. 624-634.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Adult Neural Stem Cells from the Subventricular Zone Give Rise to Reactive Astrocytes in the Cortex after Stroke

AU - Faiz, Maryam

AU - Sachewsky, Nadia

AU - Gascón, Sergio

AU - Bang, K. W.Annie

AU - Morshead, Cindi M.

AU - Nagy, Andras

PY - 2015/11/5

Y1 - 2015/11/5

N2 - Reactive astrocytes (RAs) have been reported to convert to multipotent neural stem cells (NSCs) capable of neurosphere (NS) formation and multilineage differentiation in vitro. Using genetic tagging, we determined that subventricular zone (SVZ) NSCs give rise to NSs derived from the stroke-injured cortex. We demonstrate that these cells can be isolated from the cortex in two different models of stroke and from different stroke-lesioned cortical regions. Interestingly, SVZ NSCs give rise to a subpopulation of RAs in the cortex that contribute to astrogliosis and scar formation. Last, we show that these SVZ derived RAs can be converted to neurons in vivo by forced expression of Ascl1. Identifying the contribution of cells originating from the SVZ to injury repair has implications for neural regeneration strategies.

AB - Reactive astrocytes (RAs) have been reported to convert to multipotent neural stem cells (NSCs) capable of neurosphere (NS) formation and multilineage differentiation in vitro. Using genetic tagging, we determined that subventricular zone (SVZ) NSCs give rise to NSs derived from the stroke-injured cortex. We demonstrate that these cells can be isolated from the cortex in two different models of stroke and from different stroke-lesioned cortical regions. Interestingly, SVZ NSCs give rise to a subpopulation of RAs in the cortex that contribute to astrogliosis and scar formation. Last, we show that these SVZ derived RAs can be converted to neurons in vivo by forced expression of Ascl1. Identifying the contribution of cells originating from the SVZ to injury repair has implications for neural regeneration strategies.

UR - http://www.scopus.com/inward/record.url?scp=84947813542&partnerID=8YFLogxK

U2 - 10.1016/j.stem.2015.08.002

DO - 10.1016/j.stem.2015.08.002

M3 - Article

VL - 17

SP - 624

EP - 634

JO - Cell Stem Cell

JF - Cell Stem Cell

SN - 1934-5909

IS - 5

ER -