TY - JOUR
T1 - Adsorption and Desorption of Single-Stranded DNA from Single-Walled Carbon Nanotubes
AU - Shearer, Cameron J.
AU - Yu, Le Ping
AU - Fenati, Renzo
AU - Sibley, Alexander J.
AU - Quinton, Jamie S.
AU - Gibson, Christopher T.
AU - Ellis, Amanda V.
AU - Andersson, Gunther G.
AU - Shapter, Joseph G.
N1 - Funding Information:
The authors acknowledge the facilities of the Australian Microscopy & Microanalysis Research Facility (AMMRF) at Flinders University (Raman, SEM). This work was performed in part at the South Australian node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano and micro-fabrication facilities for Australia's researchers. We wish to thank Nigel Spooner and Jillian Moffat (University of Adelaide) for access to, assistance, and expertise in photoluminescence spectroscopy. We also thank Geyou Ao, Jason Streit and Ming Zheng (National Institute of Standards and Technology, USA) for discussion on model ssDNA-SWCNT system and assistance with circular dichroism. This work was funded in part from the Australian Research Council (DP150101354).
Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/7/4
Y1 - 2017/7/4
N2 - The chemical affinity of single-stranded DNA (ssDNA) to adsorb to the surface of single-walled carbon nanotubes (SWCNTs) is used for SWCNT purification, separation and in bio-devices. Despite the popularity of research on SWCNT-ssDNA conjugates, very little work has studied the removal of adsorbed ssDNA on SWCNTs. This paper reports a comprehensive study of biological, physical and chemical treatments for the removal of ssDNA from SWCNT-ssDNA suspensions. These include enzymatic cleavage, heat treatment under vacuum up to 400 °C, chemical treatments with high or low pH, oxidizing conditions, and high-ionic-strength solvents. Complimentary characterization techniques including fluorescence from a DNA-intercalating dye (YO-PRO-1) and photoelectron spectroscopy are used to exhaustively study and compare the methods investigated. Enzyme treatment is found to remove the phosphate backbone only, leaving nucleosides adsorbed to SWCNTs. Heating in inert atmosphere is ineffective at removing ssDNA. Acid, base and oxidative treatment are found to be effective for the removal of ssDNA from SWCNTs. Where possible the mechanism of desorption is described and from the findings suggestions for “best practices” are provided.
AB - The chemical affinity of single-stranded DNA (ssDNA) to adsorb to the surface of single-walled carbon nanotubes (SWCNTs) is used for SWCNT purification, separation and in bio-devices. Despite the popularity of research on SWCNT-ssDNA conjugates, very little work has studied the removal of adsorbed ssDNA on SWCNTs. This paper reports a comprehensive study of biological, physical and chemical treatments for the removal of ssDNA from SWCNT-ssDNA suspensions. These include enzymatic cleavage, heat treatment under vacuum up to 400 °C, chemical treatments with high or low pH, oxidizing conditions, and high-ionic-strength solvents. Complimentary characterization techniques including fluorescence from a DNA-intercalating dye (YO-PRO-1) and photoelectron spectroscopy are used to exhaustively study and compare the methods investigated. Enzyme treatment is found to remove the phosphate backbone only, leaving nucleosides adsorbed to SWCNTs. Heating in inert atmosphere is ineffective at removing ssDNA. Acid, base and oxidative treatment are found to be effective for the removal of ssDNA from SWCNTs. Where possible the mechanism of desorption is described and from the findings suggestions for “best practices” are provided.
KW - carbon
KW - DNA
KW - purification methods
KW - single-walled nanotubes
UR - https://www.scopus.com/pages/publications/85019122468
U2 - 10.1002/asia.201700446
DO - 10.1002/asia.201700446
M3 - Article
C2 - 28407412
AN - SCOPUS:85019122468
SN - 1861-4728
VL - 12
SP - 1625
EP - 1634
JO - Chemistry - An Asian Journal
JF - Chemistry - An Asian Journal
IS - 13
ER -