Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 347 - 362 |
| Number of pages | 16 |
| Journal | Journal of Endocrinology |
| Volume | 221 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2014 |
Cite this
}
Adrenal steroidogenesis following prenatal dexamethasone exposure in the spiny mouse. / Quinn, Tracey Anne; Ratnayake, Udani; Castillo-Melendez, Margie Esmeralda; Moritz, Karen Margaret; Dickinson, Hayley; Walker, David William.
In: Journal of Endocrinology, Vol. 221, No. 2, 2014, p. 347 - 362.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Adrenal steroidogenesis following prenatal dexamethasone exposure in the spiny mouse
AU - Quinn, Tracey Anne
AU - Ratnayake, Udani
AU - Castillo-Melendez, Margie Esmeralda
AU - Moritz, Karen Margaret
AU - Dickinson, Hayley
AU - Walker, David William
PY - 2014
Y1 - 2014
N2 - Antenatal stress disturbs the development of the fetal hypothalamic-pituitary-adrenal axis and adrenal steroidogenesis. We investigated the effect of brief maternal exposure to high glucocorticoids (dexamethasone (DEX)) at mid- and late-pregnancy on adrenal structure and production of steroids in spiny mouse. Pregnant spiny mice were treated for 60 h with 125 mug/kg DEX or saline s.c. by osmotic minipump at day 20 (0.5) or 30 (0.75) of gestation. Immunohistochemical expression of steroidogenic acute regulatory-protein (StAR), 3beta-hydroxysteroid dehydrogenase (3betaHSD), 17-hydroxylase,17-20lyase (P450C17), and cytochromeb5 (CYTB5) was determined in adrenals on postnatal (P) day 170+/-20. DHEA, testosterone, and cortisol were measured by RIA. Maternal DEX at 20 days significantly reduced the expression of STAR, P450C17 (CYP17A1), and CYTB5 in the adrenal zona reticularis (ZR) of adult offspring, with greater change in male vs female offspring (P
AB - Antenatal stress disturbs the development of the fetal hypothalamic-pituitary-adrenal axis and adrenal steroidogenesis. We investigated the effect of brief maternal exposure to high glucocorticoids (dexamethasone (DEX)) at mid- and late-pregnancy on adrenal structure and production of steroids in spiny mouse. Pregnant spiny mice were treated for 60 h with 125 mug/kg DEX or saline s.c. by osmotic minipump at day 20 (0.5) or 30 (0.75) of gestation. Immunohistochemical expression of steroidogenic acute regulatory-protein (StAR), 3beta-hydroxysteroid dehydrogenase (3betaHSD), 17-hydroxylase,17-20lyase (P450C17), and cytochromeb5 (CYTB5) was determined in adrenals on postnatal (P) day 170+/-20. DHEA, testosterone, and cortisol were measured by RIA. Maternal DEX at 20 days significantly reduced the expression of STAR, P450C17 (CYP17A1), and CYTB5 in the adrenal zona reticularis (ZR) of adult offspring, with greater change in male vs female offspring (P
UR - http://joe.endocrinology-journals.org/content/221/2/347.full.pdf
U2 - 10.1530/JOE-13-0514
DO - 10.1530/JOE-13-0514
M3 - Article
VL - 221
SP - 347
EP - 362
JO - Journal of Endocrinology
JF - Journal of Endocrinology
SN - 0022-0795
IS - 2
ER -