Abstract
For experiments that require large numbers of preimplantation mouse embryos, such as microinjection of zygotes, gonadotropins are administered to females before mating to increase the number of oocytes that are ovulated (i.e., to induce superovulation). Pregnant mare serum gonadotropin (PMSG) is used to mimic the oocyte maturation effect of the endogenous follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG) is used to mimic the ovulation induction effect of luteinizing hormone (LH).
| Original language | English |
|---|---|
| Pages (from-to) | 24-27 |
| Number of pages | 4 |
| Journal | Cold Spring Harbor Protocols |
| Volume | 2018 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2018 |
Cite this
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Administration of gonadotropins for superovulation in mice. / Behringer, Richard; Gertsenstein, Marina; Nagy, Kristina Vintersten; Nagy, Andras.
In: Cold Spring Harbor Protocols, Vol. 2018, No. 1, 01.01.2018, p. 24-27.Research output: Contribution to journal › Article › Other › peer-review
TY - JOUR
T1 - Administration of gonadotropins for superovulation in mice
AU - Behringer, Richard
AU - Gertsenstein, Marina
AU - Nagy, Kristina Vintersten
AU - Nagy, Andras
PY - 2018/1/1
Y1 - 2018/1/1
N2 - For experiments that require large numbers of preimplantation mouse embryos, such as microinjection of zygotes, gonadotropins are administered to females before mating to increase the number of oocytes that are ovulated (i.e., to induce superovulation). Pregnant mare serum gonadotropin (PMSG) is used to mimic the oocyte maturation effect of the endogenous follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG) is used to mimic the ovulation induction effect of luteinizing hormone (LH).
AB - For experiments that require large numbers of preimplantation mouse embryos, such as microinjection of zygotes, gonadotropins are administered to females before mating to increase the number of oocytes that are ovulated (i.e., to induce superovulation). Pregnant mare serum gonadotropin (PMSG) is used to mimic the oocyte maturation effect of the endogenous follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG) is used to mimic the ovulation induction effect of luteinizing hormone (LH).
UR - http://www.scopus.com/inward/record.url?scp=85039842535&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot092403
DO - 10.1101/pdb.prot092403
M3 - Article
VL - 2018
SP - 24
EP - 27
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1940-3402
IS - 1
ER -