Abstract
Leptospirosis is one of the most widespread zoonoses in the world, and its most severe form in humans, Weil's disease,° may lead to jaundice, hemorrhage, renal failure, pulmonary hemorrhage syndrome, and sometimes,fatal multiple organ failure. Although the mechanisms underlying jaundice in leptospirosis have been gradually unraveled, the pathophysiology and distribution of leptospires during the early stage of infection are not well understood. Therefore, we investigated the hamster leptospirosis model, which is the accepted animal model of human Weil's disease, by using an in vivo imaging system to observe the whole bodies of animals infected with Leptospira interrogans and to identify the colonization and growth sites of the leptospires during the early phase of infection. Hamsters, infected subcutaneously with 104 bioluminescent leptospires, were analyzed by in vivo imaging, organ culture, and microscopy. The results showed that the luminescence from the leptospires spread through each hamster's body sequentially. The luminescence was first detected at the injection site only, and finally spread to the central abdomen, in the liver area. Additionally, the luminescence observed in the adipose tissue was the earliest detectable compared with the other organs, indicating that the leptospires colonized the adipose tissue at the early stage of leptospirosis. Adipose tissue cultures of the leptospires became positive earlier than the blood cultures. Microscopic analysis revealed that the leptospires colonized the inner walls of the blood vessels in the adipose tissue. In conclusion, this is the first study to report that adipose tissue is an important colonization site for leptospires, as demonstrated by microscopy and culture analyses of adipose tissue in the hamster model of Weil's disease.
| Original language | English |
|---|---|
| Article number | e0172973 |
| Number of pages | 13 |
| Journal | PLoS ONE |
| Volume | 12 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 28 Feb 2017 |
Keywords
- hamsters
- luminescence
- leptospira
- adipose tissue
- leptospira interrogans
- leptospirosis
- blood
- blood vessels
Cite this
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Adipose tissue is the first colonization site of Leptospira interrogans in subcutaneously infected hamsters. / Ozuru, Ryo; Saito, Mitsumasa; Kanemaru, Takaaki; Miyahara, Satoshi; Villanueva, Sharon Y.A.M.; Murray, Gerald L.; Adler, Ben; Fujii, Jun; Yoshida, Shin Ichi.
In: PLoS ONE, Vol. 12, No. 2, e0172973, 28.02.2017.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Adipose tissue is the first colonization site of Leptospira interrogans in subcutaneously infected hamsters
AU - Ozuru, Ryo
AU - Saito, Mitsumasa
AU - Kanemaru, Takaaki
AU - Miyahara, Satoshi
AU - Villanueva, Sharon Y.A.M.
AU - Murray, Gerald L.
AU - Adler, Ben
AU - Fujii, Jun
AU - Yoshida, Shin Ichi
PY - 2017/2/28
Y1 - 2017/2/28
N2 - Leptospirosis is one of the most widespread zoonoses in the world, and its most severe form in humans, Weil's disease,° may lead to jaundice, hemorrhage, renal failure, pulmonary hemorrhage syndrome, and sometimes,fatal multiple organ failure. Although the mechanisms underlying jaundice in leptospirosis have been gradually unraveled, the pathophysiology and distribution of leptospires during the early stage of infection are not well understood. Therefore, we investigated the hamster leptospirosis model, which is the accepted animal model of human Weil's disease, by using an in vivo imaging system to observe the whole bodies of animals infected with Leptospira interrogans and to identify the colonization and growth sites of the leptospires during the early phase of infection. Hamsters, infected subcutaneously with 104 bioluminescent leptospires, were analyzed by in vivo imaging, organ culture, and microscopy. The results showed that the luminescence from the leptospires spread through each hamster's body sequentially. The luminescence was first detected at the injection site only, and finally spread to the central abdomen, in the liver area. Additionally, the luminescence observed in the adipose tissue was the earliest detectable compared with the other organs, indicating that the leptospires colonized the adipose tissue at the early stage of leptospirosis. Adipose tissue cultures of the leptospires became positive earlier than the blood cultures. Microscopic analysis revealed that the leptospires colonized the inner walls of the blood vessels in the adipose tissue. In conclusion, this is the first study to report that adipose tissue is an important colonization site for leptospires, as demonstrated by microscopy and culture analyses of adipose tissue in the hamster model of Weil's disease.
AB - Leptospirosis is one of the most widespread zoonoses in the world, and its most severe form in humans, Weil's disease,° may lead to jaundice, hemorrhage, renal failure, pulmonary hemorrhage syndrome, and sometimes,fatal multiple organ failure. Although the mechanisms underlying jaundice in leptospirosis have been gradually unraveled, the pathophysiology and distribution of leptospires during the early stage of infection are not well understood. Therefore, we investigated the hamster leptospirosis model, which is the accepted animal model of human Weil's disease, by using an in vivo imaging system to observe the whole bodies of animals infected with Leptospira interrogans and to identify the colonization and growth sites of the leptospires during the early phase of infection. Hamsters, infected subcutaneously with 104 bioluminescent leptospires, were analyzed by in vivo imaging, organ culture, and microscopy. The results showed that the luminescence from the leptospires spread through each hamster's body sequentially. The luminescence was first detected at the injection site only, and finally spread to the central abdomen, in the liver area. Additionally, the luminescence observed in the adipose tissue was the earliest detectable compared with the other organs, indicating that the leptospires colonized the adipose tissue at the early stage of leptospirosis. Adipose tissue cultures of the leptospires became positive earlier than the blood cultures. Microscopic analysis revealed that the leptospires colonized the inner walls of the blood vessels in the adipose tissue. In conclusion, this is the first study to report that adipose tissue is an important colonization site for leptospires, as demonstrated by microscopy and culture analyses of adipose tissue in the hamster model of Weil's disease.
KW - hamsters
KW - luminescence
KW - leptospira
KW - adipose tissue
KW - leptospira interrogans
KW - leptospirosis
KW - blood
KW - blood vessels
UR - http://www.scopus.com/inward/record.url?scp=85014289963&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0172973
DO - 10.1371/journal.pone.0172973
M3 - Article
VL - 12
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 2
M1 - e0172973
ER -