Adequacy of a compartment model for CMRO2 quantitation using 15O-labeled oxygen and PET: a clearance measurement of 15O-radioactivity following intracarotid bolus injection of 15O-labeled oxyhemoglobin on Macaca fascicularis

Hidehiro Iida, Satoshi Iguchi, Noboru Teramoto, Kazuhiro Koshino, Tsutomu Zeniya, Akihide Yamamoto, Nobuyuki Kudomi, Tetsuaki Moriguchi, Yuki Hori, Junichiro Enmi, Hidekazu Kawashima, Nadim Joni Shah, Jyoji Nakagawara

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We aimed at evaluating the adequacy of the commonly employed compartmental model for quantitation of cerebral metabolic rate of oxygen (CMRO2) using 15O-labeled oxygen (15O2) and positron emission tomography (PET). Sequential PET imaging was carried out on monkeys following slow bolus injection of blood samples containing 15O 2-oxyhemoglobin (15O2-Hb), 15O- labeled water (H2 15O), and C15O-labeled hemoglobin (C15O-Hb) into the internal carotid artery (ICA). Clearance slopes were assessed in the middle cerebral artery territory of the injected hemisphere. The time-activity curves were bi-exponential for both 15O2-Hb and H2 15O. Single exponential fitting to the early (5 to 40 seconds) and late (80 to 240 seconds) periods after the peak was performed and the 15O2-Hb and H2 15O results were compared. It was found that a significant difference between the clearance rates of the 15O 2-Hb and H2 15O injections is unlikely, which supports the mathematical model that is widely used to describe the kinetics of 15O2-Hb and H2 15O in cerebral tissues and is the basis of recent approaches to simultaneously assess CMRO 2 and cerebral blood flow in a single PET session. However, it should be noted that more data are necessary to unequivocally confirm the result.

Original languageEnglish
Pages (from-to)1434-1439
Number of pages6
JournalJournal of Cerebral Blood Flow and Metabolism
Issue number9
Publication statusPublished - 1 Sep 2014
Externally publishedYes

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