TY - JOUR
T1 - Addressing Delicate and Variable Cancer Morphology in Spectral Histopathology Using Canine Visceral Hemangiosarcoma
AU - Oungsakul, Patharee
AU - Perez-Guaita, David
AU - Shah, Alok K.
AU - Duffy, David
AU - Wood, Bayden R.
AU - Bielefeldt-Ohmann, Helle
AU - Hill, Michelle M.
N1 - Funding Information:
P.O. was supported by Royal Thai government scholarship, the University of Queensland Top Up Assistant Program and Fred Z. Eager Research Prize in Veterinary Science. The expenses for sample preparation at School of Veterinary Science, the University of Queensland was supported by John and Mary Kibble grant. Equipment access was enabled by Australian Research Council Linkage Project LP160100731 to M.M.H. and B.R.W. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 796287. D.P.-G. acknowledges the financial support of the 2019 Ramón y Cajal (RYC) Contracts Aids (RYC2019- 026556-I). The authors thank Eunju Choi (Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis) for providing samples and feedback on the manuscript. The authors also thank Mustafa Kansiz, David Haines, and Michelle Comerford and Agilent Technologies, Australia for instrument technical support.
Publisher Copyright:
© 2021 The Authors. Published by American Chemical Society
PY - 2021/9/14
Y1 - 2021/9/14
N2 - Spectral histopathology has shown promise for the classification and diagnosis of tumors with defined morphology, but application in tumors with variable or diffuse morphologies is yet to be investigated. To address this gap, we evaluated the application of Fourier transform infrared (FTIR) imaging as an accessory diagnostic tool for canine hemangiosarcoma (HSA), a vascular endothelial cell cancer that is difficult to diagnose. To preserve the delicate vascular tumor tissue structure, and potential classification of single endothelial cells, paraffin removal was not performed, and a partial least square discrimination analysis (PLSDA) and Random Forest (RF) models to classify different tissue types at individual pixel level were established using a calibration set (24 FTIR images from 13 spleen specimens). Next, the prediction capability of the PLSDA model was tested with an independent test set (n= 11), resulting in 74% correct classification of different tissue types at an individual pixel level. Finally, the performance of the FTIR spectropathology and chemometric algorithm for diagnosis of HSA was established in a blinded set of tissue samples (n= 24), with sensitivity and specificity of 80 and 81%, respectively. Taken together, these results show that FTIR imaging without paraffin removal can be applied to tumors with diffuse morphology, and this technique is a promising tool to assist in canine splenic HSA differential diagnosis.
AB - Spectral histopathology has shown promise for the classification and diagnosis of tumors with defined morphology, but application in tumors with variable or diffuse morphologies is yet to be investigated. To address this gap, we evaluated the application of Fourier transform infrared (FTIR) imaging as an accessory diagnostic tool for canine hemangiosarcoma (HSA), a vascular endothelial cell cancer that is difficult to diagnose. To preserve the delicate vascular tumor tissue structure, and potential classification of single endothelial cells, paraffin removal was not performed, and a partial least square discrimination analysis (PLSDA) and Random Forest (RF) models to classify different tissue types at individual pixel level were established using a calibration set (24 FTIR images from 13 spleen specimens). Next, the prediction capability of the PLSDA model was tested with an independent test set (n= 11), resulting in 74% correct classification of different tissue types at an individual pixel level. Finally, the performance of the FTIR spectropathology and chemometric algorithm for diagnosis of HSA was established in a blinded set of tissue samples (n= 24), with sensitivity and specificity of 80 and 81%, respectively. Taken together, these results show that FTIR imaging without paraffin removal can be applied to tumors with diffuse morphology, and this technique is a promising tool to assist in canine splenic HSA differential diagnosis.
UR - http://www.scopus.com/inward/record.url?scp=85114459312&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.0c05190
DO - 10.1021/acs.analchem.0c05190
M3 - Article
C2 - 34459578
AN - SCOPUS:85114459312
SN - 0003-2700
VL - 93
SP - 12187
EP - 12194
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 36
ER -
