Acute epididymitis induces alterations in sperm protein composition

Adrian Pilatz, Guenter Lochnit, Srikanth Karnati, Agnieszka Paradowska-Dogan, Tali Lang, Dirk Schultheiss, Hans Christian Schuppe, Hamid Hossain, Eveline Baumgart-Vogt, Wolfgang Weidner, Florian Wagenlehner

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

Objective To use a proteomic approach to evaluate possible postinflammatory alterations in the protein composition of motile sperm in patients 3 months after acute epididymitis. Design Prospective case-control study. Setting University medical school research laboratory. Patient(s) Eight patients 3 months after acute unilateral epididymitis and 10 healthy controls. Intervention(s) None. Main Outcome Measure(s) Proteome analysis of sperm samples collected by swim-up from control and acute epididymitis patients analyzed by two-dimensional gel electrophoresis and subsequent protein identification by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry; immunofluorescence staining for mitochondrial ATP synthase subunit β (ATP5B), α-tubulin (TUBA1A), and tubulin-β2c (TUBB4B) for validation purposes. Result(s) Proteome analysis identified 35 proteins in sperm from epididymitis patients that were down-regulated, irrespective of subcellular localization and biologic function. Furthermore, immunofluorescence microscopy confirmed ATP5B, TUBA1A, and TUBB4B were less abundantly expressed in epididymitis samples compared with controls. Conclusion(s) Despite normal semen parameters observed by conventional semen analysis in patients after epididymitis, significant changes to sperm protein composition were observed. These changes may be implicated as additional factors contributing to subfertility/infertility in men after episodes of epididymitis.

Original languageEnglish
Pages (from-to)1609-1617.e5
Number of pages14
JournalFertility and Sterility
Volume101
Issue number6
DOIs
Publication statusPublished - Jun 2014
Externally publishedYes

Keywords

  • Epididymitis
  • male fertility
  • proteome analysis
  • semen quality

Cite this

Pilatz, A., Lochnit, G., Karnati, S., Paradowska-Dogan, A., Lang, T., Schultheiss, D., ... Wagenlehner, F. (2014). Acute epididymitis induces alterations in sperm protein composition. Fertility and Sterility, 101(6), 1609-1617.e5. https://doi.org/10.1016/j.fertnstert.2014.03.011
Pilatz, Adrian ; Lochnit, Guenter ; Karnati, Srikanth ; Paradowska-Dogan, Agnieszka ; Lang, Tali ; Schultheiss, Dirk ; Schuppe, Hans Christian ; Hossain, Hamid ; Baumgart-Vogt, Eveline ; Weidner, Wolfgang ; Wagenlehner, Florian. / Acute epididymitis induces alterations in sperm protein composition. In: Fertility and Sterility. 2014 ; Vol. 101, No. 6. pp. 1609-1617.e5.
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abstract = "Objective To use a proteomic approach to evaluate possible postinflammatory alterations in the protein composition of motile sperm in patients 3 months after acute epididymitis. Design Prospective case-control study. Setting University medical school research laboratory. Patient(s) Eight patients 3 months after acute unilateral epididymitis and 10 healthy controls. Intervention(s) None. Main Outcome Measure(s) Proteome analysis of sperm samples collected by swim-up from control and acute epididymitis patients analyzed by two-dimensional gel electrophoresis and subsequent protein identification by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry; immunofluorescence staining for mitochondrial ATP synthase subunit β (ATP5B), α-tubulin (TUBA1A), and tubulin-β2c (TUBB4B) for validation purposes. Result(s) Proteome analysis identified 35 proteins in sperm from epididymitis patients that were down-regulated, irrespective of subcellular localization and biologic function. Furthermore, immunofluorescence microscopy confirmed ATP5B, TUBA1A, and TUBB4B were less abundantly expressed in epididymitis samples compared with controls. Conclusion(s) Despite normal semen parameters observed by conventional semen analysis in patients after epididymitis, significant changes to sperm protein composition were observed. These changes may be implicated as additional factors contributing to subfertility/infertility in men after episodes of epididymitis.",
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author = "Adrian Pilatz and Guenter Lochnit and Srikanth Karnati and Agnieszka Paradowska-Dogan and Tali Lang and Dirk Schultheiss and Schuppe, {Hans Christian} and Hamid Hossain and Eveline Baumgart-Vogt and Wolfgang Weidner and Florian Wagenlehner",
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Pilatz, A, Lochnit, G, Karnati, S, Paradowska-Dogan, A, Lang, T, Schultheiss, D, Schuppe, HC, Hossain, H, Baumgart-Vogt, E, Weidner, W & Wagenlehner, F 2014, 'Acute epididymitis induces alterations in sperm protein composition', Fertility and Sterility, vol. 101, no. 6, pp. 1609-1617.e5. https://doi.org/10.1016/j.fertnstert.2014.03.011

Acute epididymitis induces alterations in sperm protein composition. / Pilatz, Adrian; Lochnit, Guenter; Karnati, Srikanth; Paradowska-Dogan, Agnieszka; Lang, Tali; Schultheiss, Dirk; Schuppe, Hans Christian; Hossain, Hamid; Baumgart-Vogt, Eveline; Weidner, Wolfgang; Wagenlehner, Florian.

In: Fertility and Sterility, Vol. 101, No. 6, 06.2014, p. 1609-1617.e5.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Acute epididymitis induces alterations in sperm protein composition

AU - Pilatz, Adrian

AU - Lochnit, Guenter

AU - Karnati, Srikanth

AU - Paradowska-Dogan, Agnieszka

AU - Lang, Tali

AU - Schultheiss, Dirk

AU - Schuppe, Hans Christian

AU - Hossain, Hamid

AU - Baumgart-Vogt, Eveline

AU - Weidner, Wolfgang

AU - Wagenlehner, Florian

PY - 2014/6

Y1 - 2014/6

N2 - Objective To use a proteomic approach to evaluate possible postinflammatory alterations in the protein composition of motile sperm in patients 3 months after acute epididymitis. Design Prospective case-control study. Setting University medical school research laboratory. Patient(s) Eight patients 3 months after acute unilateral epididymitis and 10 healthy controls. Intervention(s) None. Main Outcome Measure(s) Proteome analysis of sperm samples collected by swim-up from control and acute epididymitis patients analyzed by two-dimensional gel electrophoresis and subsequent protein identification by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry; immunofluorescence staining for mitochondrial ATP synthase subunit β (ATP5B), α-tubulin (TUBA1A), and tubulin-β2c (TUBB4B) for validation purposes. Result(s) Proteome analysis identified 35 proteins in sperm from epididymitis patients that were down-regulated, irrespective of subcellular localization and biologic function. Furthermore, immunofluorescence microscopy confirmed ATP5B, TUBA1A, and TUBB4B were less abundantly expressed in epididymitis samples compared with controls. Conclusion(s) Despite normal semen parameters observed by conventional semen analysis in patients after epididymitis, significant changes to sperm protein composition were observed. These changes may be implicated as additional factors contributing to subfertility/infertility in men after episodes of epididymitis.

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KW - Epididymitis

KW - male fertility

KW - proteome analysis

KW - semen quality

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U2 - 10.1016/j.fertnstert.2014.03.011

DO - 10.1016/j.fertnstert.2014.03.011

M3 - Article

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SN - 0015-0282

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Pilatz A, Lochnit G, Karnati S, Paradowska-Dogan A, Lang T, Schultheiss D et al. Acute epididymitis induces alterations in sperm protein composition. Fertility and Sterility. 2014 Jun;101(6):1609-1617.e5. https://doi.org/10.1016/j.fertnstert.2014.03.011