Acute effect of endothelins on intercellular communication of human embryonic stem cells

Raymond C.B. Wong; Kathryn C. Davidson; Jessie Leung; Martin F. Pera; Alice Pébay

Acute effect of endothelins on intercellular communication of human embryonic stem cells

Raymond C.B. Wong, Kathryn C. Davidson, Jessie Leung, Martin F. Pera, Alice Pébay

Hudson Institute - Department of Molecular and Translational Science

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

Endothelin (ET) family comprises three isoforms, ET-1, ET-2 and ET-3 that bind to two receptors ET-A and ET-B. Upon hESC differentiation, ET-1 and ET-B are respectively up- and down-regulated, suggesting a potential role of ETs in hESC biology. Here we show expression of ET receptors in hESC and demonstrate that ET-1 and ET-2 inhibit gap junctional intercellular communication (GJIC), while ET-3 does not. Pre-incubation of the cell cultures with the two specific antagonists of ET-A and ET-B, BQ123 and BQ788 respectively, demonstrate that inhibition of GJIC by ETs is mediated by ET-A. Long-term treatment of hESC with ET-1 indicates no visible effect on hESC maintenance of pluripotency markers, as assessed by expression of the hESC markers Oct-4, GCTM-2 and TG-30. Altogether these data show that hESC are target cells of ETs.

Original language	English
Pages (from-to)	47-56
Number of pages	10
Journal	Journal of Stem Cells
Volume	4
Issue number	1
Publication status	Published - 2009

Keywords

Endothelin
Gap junction
Gap junctional intercellular communication
Human embryonic stem cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Acute effect of endothelins on intercellular communication of human embryonic stem cells",

abstract = "Endothelin (ET) family comprises three isoforms, ET-1, ET-2 and ET-3 that bind to two receptors ET-A and ET-B. Upon hESC differentiation, ET-1 and ET-B are respectively up- and down-regulated, suggesting a potential role of ETs in hESC biology. Here we show expression of ET receptors in hESC and demonstrate that ET-1 and ET-2 inhibit gap junctional intercellular communication (GJIC), while ET-3 does not. Pre-incubation of the cell cultures with the two specific antagonists of ET-A and ET-B, BQ123 and BQ788 respectively, demonstrate that inhibition of GJIC by ETs is mediated by ET-A. Long-term treatment of hESC with ET-1 indicates no visible effect on hESC maintenance of pluripotency markers, as assessed by expression of the hESC markers Oct-4, GCTM-2 and TG-30. Altogether these data show that hESC are target cells of ETs.",

keywords = "Endothelin, Gap junction, Gap junctional intercellular communication, Human embryonic stem cells",

author = "Wong, {Raymond C.B.} and Davidson, {Kathryn C.} and Jessie Leung and Pera, {Martin F.} and Alice P{\'e}bay",

year = "2009",

language = "English",

volume = "4",

pages = "47--56",

journal = "Journal of Stem Cells",

issn = "1556-8539",

publisher = "Nova Science Publishers",

number = "1",

}

TY - JOUR

T1 - Acute effect of endothelins on intercellular communication of human embryonic stem cells

AU - Wong, Raymond C.B.

AU - Davidson, Kathryn C.

AU - Leung, Jessie

AU - Pera, Martin F.

AU - Pébay, Alice

PY - 2009

Y1 - 2009

N2 - Endothelin (ET) family comprises three isoforms, ET-1, ET-2 and ET-3 that bind to two receptors ET-A and ET-B. Upon hESC differentiation, ET-1 and ET-B are respectively up- and down-regulated, suggesting a potential role of ETs in hESC biology. Here we show expression of ET receptors in hESC and demonstrate that ET-1 and ET-2 inhibit gap junctional intercellular communication (GJIC), while ET-3 does not. Pre-incubation of the cell cultures with the two specific antagonists of ET-A and ET-B, BQ123 and BQ788 respectively, demonstrate that inhibition of GJIC by ETs is mediated by ET-A. Long-term treatment of hESC with ET-1 indicates no visible effect on hESC maintenance of pluripotency markers, as assessed by expression of the hESC markers Oct-4, GCTM-2 and TG-30. Altogether these data show that hESC are target cells of ETs.

AB - Endothelin (ET) family comprises three isoforms, ET-1, ET-2 and ET-3 that bind to two receptors ET-A and ET-B. Upon hESC differentiation, ET-1 and ET-B are respectively up- and down-regulated, suggesting a potential role of ETs in hESC biology. Here we show expression of ET receptors in hESC and demonstrate that ET-1 and ET-2 inhibit gap junctional intercellular communication (GJIC), while ET-3 does not. Pre-incubation of the cell cultures with the two specific antagonists of ET-A and ET-B, BQ123 and BQ788 respectively, demonstrate that inhibition of GJIC by ETs is mediated by ET-A. Long-term treatment of hESC with ET-1 indicates no visible effect on hESC maintenance of pluripotency markers, as assessed by expression of the hESC markers Oct-4, GCTM-2 and TG-30. Altogether these data show that hESC are target cells of ETs.

KW - Endothelin

KW - Gap junction

KW - Gap junctional intercellular communication

KW - Human embryonic stem cells

UR - http://www.scopus.com/inward/record.url?scp=77955240469&partnerID=8YFLogxK

M3 - Article

C2 - 20498690

AN - SCOPUS:77955240469

VL - 4

SP - 47

EP - 56

JO - Journal of Stem Cells

JF - Journal of Stem Cells

SN - 1556-8539

IS - 1

ER -

Acute effect of endothelins on intercellular communication of human embryonic stem cells

Abstract

Keywords

UN SDGs

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Research output

Acute effect of endothelins on intercellular communication of human embryonic stem cells

Cite this