Research output per year
Research output per year
Raymond C.B. Wong, Kathryn C. Davidson, Jessie Leung, Martin F. Pera, Alice Pébay
Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other
Endothelin (ET) family comprises three isoforms, ET-1, ET-2 and ET-3 that bind to two receptors ET-A and ET-B. Upon hESC differentiation, ET-1 and ET-B are respectively up- and down-regulated, suggesting a potential role of ETs in hESC biology. Here we show expression of ET receptors in hESC and demonstrate that ET-1 and ET-2 inhibit gap junctional intercellular communication (GJIC), while ET-3 does not. Pre-incubation of the cell cultures with the two specific antagonists of ET-A and ET-B, BQ123 and BQ788 respectively, demonstrate that inhibition of GJIC by ETs is mediated by ET-A. Long-term treatment of hESC with ET-1 indicates no visible effect on hESC maintenance of pluripotency markers, as assessed by expression of the hESC markers Oct-4, GCTM-2 and TG-30. Altogether these data show that hESC are target cells of ETs.
Original language | English |
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Title of host publication | Human Mesenchymal and Embryonic Stem Cells |
Editors | Prasad S. Koka |
Place of Publication | United States |
Publisher | Nova Science Publishers |
Pages | 233-246 |
Number of pages | 14 |
ISBN (Print) | 9781613240045, 9781620819098 |
Publication status | Published - 2011 |
Name | Nova Biomedical |
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Publisher | Nova Science Publishers |
Name | Cell Biology Research Progress |
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Publisher | Nova Science Publishers |
Research output: Contribution to journal › Article › Research › peer-review