Active Micropump-Mixer for Rapid Antiplatelet Drug Screening in Whole Blood

Crispin Szydzik, Rose J. Brazilek, Farzan Akbaridoust, Charitha De Silva, Mitchell Moon, Ivan Marusic, Andrew S.H. Ooi, Harshal H. Nandurkar, Justin R. Hamilton, Arnan Mitchell, Warwick S. Nesbitt

Research output: Contribution to journalArticleResearchpeer-review

Abstract

There is a need for scalable automated lab-on-chip systems incorporating precise hemodynamic control that can be applied to high-content screening of new more efficacious antiplatelet therapies. This paper reports on the development and characterization of a novel active micropump-mixer microfluidic to address this need. Using a novel reciprocating elastomeric micropump design, we take advantage of the flexible structural and actuation properties of this framework to manage the hemodynamics for on-chip platelet thrombosis assay on type 1 fibrillar collagen, using whole blood. By characterizing and harnessing the complex three-dimensional hemodynamics of the micropump operation in conjunction with a microvalve controlled reagent injection system we demonstrate that this prototype can act as a real-time assay of antiplatelet drug pharmacokinetics. In a proof-of-concept preclinical application, we utilize this system to investigate the way in which rapid dosing of human whole blood with isoform selective inhibitors of phosphatidylinositol 3-kinase dose dependently modulate platelet thrombus dynamics. This modular system exhibits utility as an automated multiplexable assay system with applications to high-content chemical library screening of new antiplatelet therapies. ©

Original languageEnglish
Pages (from-to)10830-10839
Number of pages10
JournalAnalytical Chemistry
Volume91
Issue number16
DOIs
Publication statusPublished - 20 Aug 2019

Cite this

Szydzik, Crispin ; Brazilek, Rose J. ; Akbaridoust, Farzan ; De Silva, Charitha ; Moon, Mitchell ; Marusic, Ivan ; Ooi, Andrew S.H. ; Nandurkar, Harshal H. ; Hamilton, Justin R. ; Mitchell, Arnan ; Nesbitt, Warwick S. / Active Micropump-Mixer for Rapid Antiplatelet Drug Screening in Whole Blood. In: Analytical Chemistry. 2019 ; Vol. 91, No. 16. pp. 10830-10839.
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Szydzik, C, Brazilek, RJ, Akbaridoust, F, De Silva, C, Moon, M, Marusic, I, Ooi, ASH, Nandurkar, HH, Hamilton, JR, Mitchell, A & Nesbitt, WS 2019, 'Active Micropump-Mixer for Rapid Antiplatelet Drug Screening in Whole Blood' Analytical Chemistry, vol. 91, no. 16, pp. 10830-10839. https://doi.org/10.1021/acs.analchem.9b02486

Active Micropump-Mixer for Rapid Antiplatelet Drug Screening in Whole Blood. / Szydzik, Crispin; Brazilek, Rose J.; Akbaridoust, Farzan; De Silva, Charitha; Moon, Mitchell; Marusic, Ivan; Ooi, Andrew S.H.; Nandurkar, Harshal H.; Hamilton, Justin R.; Mitchell, Arnan; Nesbitt, Warwick S.

In: Analytical Chemistry, Vol. 91, No. 16, 20.08.2019, p. 10830-10839.

Research output: Contribution to journalArticleResearchpeer-review

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Szydzik C, Brazilek RJ, Akbaridoust F, De Silva C, Moon M, Marusic I et al. Active Micropump-Mixer for Rapid Antiplatelet Drug Screening in Whole Blood. Analytical Chemistry. 2019 Aug 20;91(16):10830-10839. https://doi.org/10.1021/acs.analchem.9b02486