Active immunization of ewes against luteinizing hormone releasing hormone, and its effects on ovulation and gonadotrophin, prolactin and ovarian steroid secretion

I. J. Clarke, H. M. Fraser, A. S. McNeilly

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Abstract

Three Scottish Blackface ewes were immunized against luteinizing hormone releasing hormone (LH-RH) conjugated to bovine serum albumin (BSA) and three control ewes were immunized against BSA alone. When the antibody titre to LH-RH became raised the treated animals failed to show oestrus or ovulate; they had significantly lower levels of plasma luteinizing hormone (LH) and higher levels of prolactin than the controls, whereas the levels of follicle-stimulating hormone (FSH) were unaltered. The integrity of the hypothalamus-pituitary-gonadal system of these animals was then challenged by the injection of a highly active analogue of LH-RH and by ovariectomy. An i.v. injection of 5 μg D-serine-t-butyl6 des-glycine-NH210 LH-RH ethylamide raised plasma LH and FSH. Ovariectomy caused an eight- and ninefold rise in plasma levels of LH and FSH respectively in controls, but failed to increase plasma levels of LH and FSH in the LH-RH-immunized ewes. Plasma prolactin concentrations in the LH-RH immunized ewes were significantly reduced by ovariectomy.

Original languageEnglish
Pages (from-to)39-47
Number of pages9
JournalJournal of Endocrinology
Volume78
Issue number1
DOIs
Publication statusPublished - 1 Jan 1978
Externally publishedYes

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