Activation of cAMP-dependent protein kinase increases the protein level of steroidogenic factor-1

Reidun Aesoey, Gunnar Mellgren, Ken-Ichirou Morohashi, Johan Lund

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)

Abstract

The orphan nuclear receptor steroidogenic factor 1 (SF-1) is an essential regulator of endocrine organogenesis, sexual differentiation, and steroidogenesis. SF-1 is a transcriptional regulator of cAMP responsive genes, but the exact mechanisms by which cAMP-dependent PKA modulates SF-1 dependent transcription leading to increased steroidogenic output have not been determined. In this report the effects of PKA activation on SF-1 in living cells have been examined by the use of full-length SF-1 cDNA fused to the cDNA encoding green fluorescent protein (GFP). The GFP-SF-1 fusion protein localized to the nucleus of both steroidogenic Y1 cells and nonsteroidogenic COS-1 cells, and the functional properties of wild-type SF-1 were conserved. When the catalytic subunit of PKA was coexpressed with GFP-SF-1, we observed that the fluorescence emission was markedly elevated. These findings were confirmed by Western blot analysis, showing that stimulation of PKA increased SF-1 protein levels. The PKA- induced expression of SF-1 protein was not accompanied by an increase in SF-1 mRNA levels. However, pulse-chase studies showed a decrease in SF-1 degradation rate in response to activation of PKA, indicating that PKA elevates the level of SF-1 by increasing the stability of SF-1 protein.
Original languageEnglish
Pages (from-to)295 - 303
Number of pages9
JournalEndocrinology
Volume143
Issue number1
Publication statusPublished - 2002
Externally publishedYes

Cite this

Aesoey, R., Mellgren, G., Morohashi, K-I., & Lund, J. (2002). Activation of cAMP-dependent protein kinase increases the protein level of steroidogenic factor-1. Endocrinology, 143(1), 295 - 303.
Aesoey, Reidun ; Mellgren, Gunnar ; Morohashi, Ken-Ichirou ; Lund, Johan. / Activation of cAMP-dependent protein kinase increases the protein level of steroidogenic factor-1. In: Endocrinology. 2002 ; Vol. 143, No. 1. pp. 295 - 303.
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abstract = "The orphan nuclear receptor steroidogenic factor 1 (SF-1) is an essential regulator of endocrine organogenesis, sexual differentiation, and steroidogenesis. SF-1 is a transcriptional regulator of cAMP responsive genes, but the exact mechanisms by which cAMP-dependent PKA modulates SF-1 dependent transcription leading to increased steroidogenic output have not been determined. In this report the effects of PKA activation on SF-1 in living cells have been examined by the use of full-length SF-1 cDNA fused to the cDNA encoding green fluorescent protein (GFP). The GFP-SF-1 fusion protein localized to the nucleus of both steroidogenic Y1 cells and nonsteroidogenic COS-1 cells, and the functional properties of wild-type SF-1 were conserved. When the catalytic subunit of PKA was coexpressed with GFP-SF-1, we observed that the fluorescence emission was markedly elevated. These findings were confirmed by Western blot analysis, showing that stimulation of PKA increased SF-1 protein levels. The PKA- induced expression of SF-1 protein was not accompanied by an increase in SF-1 mRNA levels. However, pulse-chase studies showed a decrease in SF-1 degradation rate in response to activation of PKA, indicating that PKA elevates the level of SF-1 by increasing the stability of SF-1 protein.",
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Aesoey, R, Mellgren, G, Morohashi, K-I & Lund, J 2002, 'Activation of cAMP-dependent protein kinase increases the protein level of steroidogenic factor-1', Endocrinology, vol. 143, no. 1, pp. 295 - 303.

Activation of cAMP-dependent protein kinase increases the protein level of steroidogenic factor-1. / Aesoey, Reidun; Mellgren, Gunnar; Morohashi, Ken-Ichirou; Lund, Johan.

In: Endocrinology, Vol. 143, No. 1, 2002, p. 295 - 303.

Research output: Contribution to journalArticleResearchpeer-review

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