Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses

Nicole M D van der Burg, Stéphane Chappaz, Anne Baerenwaldt, Edit Horvath, Somdeb Bose Dasgupta, Devika Ashok, Jean Pieters, Fabienne Tacchini-Cottier, Antonius G Rolink, Hans Acha-Orbea, Daniela Finke

Research output: Contribution to journalArticleResearchpeer-review

71 Citations (Scopus)

Abstract

Group 3 innate lymphoid cells (ILC3s) have emerged as important cellular players in tissue repair and innate immunity. Whether these cells meaningfully regulate adaptive immune responses upon activation has yet to be explored. Here we show that upon IL- 1β stimulation, peripheral ILC3s become activated, secrete cytokines, up-regulate surface MHC class II molecules, and express costimulatory molecules. ILC3s can take up latex beads, process protein antigen, and consequently prime CD4+ T-cell responses in vitro. The cognate interaction of ILC3s and CD4+ T cells leads to T-cell proliferation both in vitro and in vivo, whereas its disruption impairs specific T-cell and T-dependent B-cell responses in vivo. In addition, the ILC3-CD4+ T-cell interaction is bidirectional and leads to the activation of ILC3s. Taken together, our data reveal a novel activation-dependent function of peripheral ILC3s in eliciting cognate CD4+ T-cell immune responses.

Original languageEnglish
Pages (from-to)12835-12840
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume111
Issue number35
DOIs
Publication statusPublished - 2 Sep 2014
Externally publishedYes

Keywords

  • Antigen presentation
  • T-cell activation

Cite this

van der Burg, N. M. D., Chappaz, S., Baerenwaldt, A., Horvath, E., Bose Dasgupta, S., Ashok, D., ... Finke, D. (2014). Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses. Proceedings of the National Academy of Sciences, 111(35), 12835-12840. https://doi.org/10.1073/pnas.1406908111
van der Burg, Nicole M D ; Chappaz, Stéphane ; Baerenwaldt, Anne ; Horvath, Edit ; Bose Dasgupta, Somdeb ; Ashok, Devika ; Pieters, Jean ; Tacchini-Cottier, Fabienne ; Rolink, Antonius G ; Acha-Orbea, Hans ; Finke, Daniela. / Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses. In: Proceedings of the National Academy of Sciences. 2014 ; Vol. 111, No. 35. pp. 12835-12840.
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van der Burg, NMD, Chappaz, S, Baerenwaldt, A, Horvath, E, Bose Dasgupta, S, Ashok, D, Pieters, J, Tacchini-Cottier, F, Rolink, AG, Acha-Orbea, H & Finke, D 2014, 'Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses', Proceedings of the National Academy of Sciences, vol. 111, no. 35, pp. 12835-12840. https://doi.org/10.1073/pnas.1406908111

Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses. / van der Burg, Nicole M D; Chappaz, Stéphane; Baerenwaldt, Anne; Horvath, Edit; Bose Dasgupta, Somdeb; Ashok, Devika; Pieters, Jean; Tacchini-Cottier, Fabienne; Rolink, Antonius G; Acha-Orbea, Hans; Finke, Daniela.

In: Proceedings of the National Academy of Sciences, Vol. 111, No. 35, 02.09.2014, p. 12835-12840.

Research output: Contribution to journalArticleResearchpeer-review

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AU - van der Burg, Nicole M D

AU - Chappaz, Stéphane

AU - Baerenwaldt, Anne

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AU - Bose Dasgupta, Somdeb

AU - Ashok, Devika

AU - Pieters, Jean

AU - Tacchini-Cottier, Fabienne

AU - Rolink, Antonius G

AU - Acha-Orbea, Hans

AU - Finke, Daniela

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N2 - Group 3 innate lymphoid cells (ILC3s) have emerged as important cellular players in tissue repair and innate immunity. Whether these cells meaningfully regulate adaptive immune responses upon activation has yet to be explored. Here we show that upon IL- 1β stimulation, peripheral ILC3s become activated, secrete cytokines, up-regulate surface MHC class II molecules, and express costimulatory molecules. ILC3s can take up latex beads, process protein antigen, and consequently prime CD4+ T-cell responses in vitro. The cognate interaction of ILC3s and CD4+ T cells leads to T-cell proliferation both in vitro and in vivo, whereas its disruption impairs specific T-cell and T-dependent B-cell responses in vivo. In addition, the ILC3-CD4+ T-cell interaction is bidirectional and leads to the activation of ILC3s. Taken together, our data reveal a novel activation-dependent function of peripheral ILC3s in eliciting cognate CD4+ T-cell immune responses.

AB - Group 3 innate lymphoid cells (ILC3s) have emerged as important cellular players in tissue repair and innate immunity. Whether these cells meaningfully regulate adaptive immune responses upon activation has yet to be explored. Here we show that upon IL- 1β stimulation, peripheral ILC3s become activated, secrete cytokines, up-regulate surface MHC class II molecules, and express costimulatory molecules. ILC3s can take up latex beads, process protein antigen, and consequently prime CD4+ T-cell responses in vitro. The cognate interaction of ILC3s and CD4+ T cells leads to T-cell proliferation both in vitro and in vivo, whereas its disruption impairs specific T-cell and T-dependent B-cell responses in vivo. In addition, the ILC3-CD4+ T-cell interaction is bidirectional and leads to the activation of ILC3s. Taken together, our data reveal a novel activation-dependent function of peripheral ILC3s in eliciting cognate CD4+ T-cell immune responses.

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