Abstract
Group 3 innate lymphoid cells (ILC3s) have emerged as important cellular players in tissue repair and innate immunity. Whether these cells meaningfully regulate adaptive immune responses upon activation has yet to be explored. Here we show that upon IL- 1β stimulation, peripheral ILC3s become activated, secrete cytokines, up-regulate surface MHC class II molecules, and express costimulatory molecules. ILC3s can take up latex beads, process protein antigen, and consequently prime CD4+ T-cell responses in vitro. The cognate interaction of ILC3s and CD4+ T cells leads to T-cell proliferation both in vitro and in vivo, whereas its disruption impairs specific T-cell and T-dependent B-cell responses in vivo. In addition, the ILC3-CD4+ T-cell interaction is bidirectional and leads to the activation of ILC3s. Taken together, our data reveal a novel activation-dependent function of peripheral ILC3s in eliciting cognate CD4+ T-cell immune responses.
| Original language | English |
|---|---|
| Pages (from-to) | 12835-12840 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences |
| Volume | 111 |
| Issue number | 35 |
| DOIs | |
| Publication status | Published - 2 Sep 2014 |
| Externally published | Yes |
Keywords
- Antigen presentation
- T-cell activation
Cite this
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Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses. / van der Burg, Nicole M D; Chappaz, Stéphane; Baerenwaldt, Anne; Horvath, Edit; Bose Dasgupta, Somdeb; Ashok, Devika; Pieters, Jean; Tacchini-Cottier, Fabienne; Rolink, Antonius G; Acha-Orbea, Hans; Finke, Daniela.
In: Proceedings of the National Academy of Sciences, Vol. 111, No. 35, 02.09.2014, p. 12835-12840.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses
AU - van der Burg, Nicole M D
AU - Chappaz, Stéphane
AU - Baerenwaldt, Anne
AU - Horvath, Edit
AU - Bose Dasgupta, Somdeb
AU - Ashok, Devika
AU - Pieters, Jean
AU - Tacchini-Cottier, Fabienne
AU - Rolink, Antonius G
AU - Acha-Orbea, Hans
AU - Finke, Daniela
PY - 2014/9/2
Y1 - 2014/9/2
N2 - Group 3 innate lymphoid cells (ILC3s) have emerged as important cellular players in tissue repair and innate immunity. Whether these cells meaningfully regulate adaptive immune responses upon activation has yet to be explored. Here we show that upon IL- 1β stimulation, peripheral ILC3s become activated, secrete cytokines, up-regulate surface MHC class II molecules, and express costimulatory molecules. ILC3s can take up latex beads, process protein antigen, and consequently prime CD4+ T-cell responses in vitro. The cognate interaction of ILC3s and CD4+ T cells leads to T-cell proliferation both in vitro and in vivo, whereas its disruption impairs specific T-cell and T-dependent B-cell responses in vivo. In addition, the ILC3-CD4+ T-cell interaction is bidirectional and leads to the activation of ILC3s. Taken together, our data reveal a novel activation-dependent function of peripheral ILC3s in eliciting cognate CD4+ T-cell immune responses.
AB - Group 3 innate lymphoid cells (ILC3s) have emerged as important cellular players in tissue repair and innate immunity. Whether these cells meaningfully regulate adaptive immune responses upon activation has yet to be explored. Here we show that upon IL- 1β stimulation, peripheral ILC3s become activated, secrete cytokines, up-regulate surface MHC class II molecules, and express costimulatory molecules. ILC3s can take up latex beads, process protein antigen, and consequently prime CD4+ T-cell responses in vitro. The cognate interaction of ILC3s and CD4+ T cells leads to T-cell proliferation both in vitro and in vivo, whereas its disruption impairs specific T-cell and T-dependent B-cell responses in vivo. In addition, the ILC3-CD4+ T-cell interaction is bidirectional and leads to the activation of ILC3s. Taken together, our data reveal a novel activation-dependent function of peripheral ILC3s in eliciting cognate CD4+ T-cell immune responses.
KW - Antigen presentation
KW - T-cell activation
UR - http://www.scopus.com/inward/record.url?scp=84907228164&partnerID=8YFLogxK
U2 - 10.1073/pnas.1406908111
DO - 10.1073/pnas.1406908111
M3 - Article
VL - 111
SP - 12835
EP - 12840
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 35
ER -