Acrylic-acid-functionalized polyHIPE scaffolds for use in 3D cell culture

Adam S. Hayward, Naoko Sano, Stefan A. Przyborski, Neil R. Cameron

Research output: Contribution to journalArticleResearchpeer-review

33 Citations (Scopus)

Abstract

This study describes the development of a functional porous polymer for use as a scaffold to support 3D hepatocyte culture. A high internal phase emulsion (HIPE) is prepared containing the monomers styrene (STY), divinylbenzene (DVB), and 2-ethylhexyl acrylate (EHA) in the external oil phase and the monomer acrylic acid (Aa) in the internal aqueous phase. Upon thermal polymerization with azobisisobutyronitrile (AIBN), the resulting porous polymer (polyHIPE) is found to have an open-cell morphology and a porosity of 89%, both suitable characteristics for 3D cell scaffold applications. X-ray photoelectron spectroscopy reveals that the polyHIPE surface contained 7.5% carboxylic acid functionality, providing a useful substrate for subsequent surface modifi cations and bio-conjugations. Initial bio-compatibility assessments with human hepatocytes show that the acid functionality does not have any detrimental effect on cell adhesion. It is therefore believed that this material can be a useful precursor scaffold towards 3D substrates that offer tailored surface functionality for enhanced cell adhesion.

Original languageEnglish
Pages (from-to)1844-1849
Number of pages6
JournalMacromolecular Rapid Communications
Volume34
Issue number23-24
DOIs
Publication statusPublished - Dec 2013
Externally publishedYes

Keywords

  • 3D cell culture
  • Acrylic acid
  • PolyHIPE
  • Polystyrene
  • Scaffold

Cite this

Hayward, Adam S. ; Sano, Naoko ; Przyborski, Stefan A. ; Cameron, Neil R. / Acrylic-acid-functionalized polyHIPE scaffolds for use in 3D cell culture. In: Macromolecular Rapid Communications. 2013 ; Vol. 34, No. 23-24. pp. 1844-1849.
@article{4fa41c3fa62d41aeb368d41ab316239a,
title = "Acrylic-acid-functionalized polyHIPE scaffolds for use in 3D cell culture",
abstract = "This study describes the development of a functional porous polymer for use as a scaffold to support 3D hepatocyte culture. A high internal phase emulsion (HIPE) is prepared containing the monomers styrene (STY), divinylbenzene (DVB), and 2-ethylhexyl acrylate (EHA) in the external oil phase and the monomer acrylic acid (Aa) in the internal aqueous phase. Upon thermal polymerization with azobisisobutyronitrile (AIBN), the resulting porous polymer (polyHIPE) is found to have an open-cell morphology and a porosity of 89{\%}, both suitable characteristics for 3D cell scaffold applications. X-ray photoelectron spectroscopy reveals that the polyHIPE surface contained 7.5{\%} carboxylic acid functionality, providing a useful substrate for subsequent surface modifi cations and bio-conjugations. Initial bio-compatibility assessments with human hepatocytes show that the acid functionality does not have any detrimental effect on cell adhesion. It is therefore believed that this material can be a useful precursor scaffold towards 3D substrates that offer tailored surface functionality for enhanced cell adhesion.",
keywords = "3D cell culture, Acrylic acid, PolyHIPE, Polystyrene, Scaffold",
author = "Hayward, {Adam S.} and Naoko Sano and Przyborski, {Stefan A.} and Cameron, {Neil R.}",
year = "2013",
month = "12",
doi = "10.1002/marc.201300709",
language = "English",
volume = "34",
pages = "1844--1849",
journal = "Macromolecular Rapid Communications",
issn = "1022-1336",
publisher = "H{\"u}thig & Wepf Verlag",
number = "23-24",

}

Acrylic-acid-functionalized polyHIPE scaffolds for use in 3D cell culture. / Hayward, Adam S.; Sano, Naoko; Przyborski, Stefan A.; Cameron, Neil R.

In: Macromolecular Rapid Communications, Vol. 34, No. 23-24, 12.2013, p. 1844-1849.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Acrylic-acid-functionalized polyHIPE scaffolds for use in 3D cell culture

AU - Hayward, Adam S.

AU - Sano, Naoko

AU - Przyborski, Stefan A.

AU - Cameron, Neil R.

PY - 2013/12

Y1 - 2013/12

N2 - This study describes the development of a functional porous polymer for use as a scaffold to support 3D hepatocyte culture. A high internal phase emulsion (HIPE) is prepared containing the monomers styrene (STY), divinylbenzene (DVB), and 2-ethylhexyl acrylate (EHA) in the external oil phase and the monomer acrylic acid (Aa) in the internal aqueous phase. Upon thermal polymerization with azobisisobutyronitrile (AIBN), the resulting porous polymer (polyHIPE) is found to have an open-cell morphology and a porosity of 89%, both suitable characteristics for 3D cell scaffold applications. X-ray photoelectron spectroscopy reveals that the polyHIPE surface contained 7.5% carboxylic acid functionality, providing a useful substrate for subsequent surface modifi cations and bio-conjugations. Initial bio-compatibility assessments with human hepatocytes show that the acid functionality does not have any detrimental effect on cell adhesion. It is therefore believed that this material can be a useful precursor scaffold towards 3D substrates that offer tailored surface functionality for enhanced cell adhesion.

AB - This study describes the development of a functional porous polymer for use as a scaffold to support 3D hepatocyte culture. A high internal phase emulsion (HIPE) is prepared containing the monomers styrene (STY), divinylbenzene (DVB), and 2-ethylhexyl acrylate (EHA) in the external oil phase and the monomer acrylic acid (Aa) in the internal aqueous phase. Upon thermal polymerization with azobisisobutyronitrile (AIBN), the resulting porous polymer (polyHIPE) is found to have an open-cell morphology and a porosity of 89%, both suitable characteristics for 3D cell scaffold applications. X-ray photoelectron spectroscopy reveals that the polyHIPE surface contained 7.5% carboxylic acid functionality, providing a useful substrate for subsequent surface modifi cations and bio-conjugations. Initial bio-compatibility assessments with human hepatocytes show that the acid functionality does not have any detrimental effect on cell adhesion. It is therefore believed that this material can be a useful precursor scaffold towards 3D substrates that offer tailored surface functionality for enhanced cell adhesion.

KW - 3D cell culture

KW - Acrylic acid

KW - PolyHIPE

KW - Polystyrene

KW - Scaffold

UR - http://www.scopus.com/inward/record.url?scp=84894306245&partnerID=8YFLogxK

U2 - 10.1002/marc.201300709

DO - 10.1002/marc.201300709

M3 - Article

VL - 34

SP - 1844

EP - 1849

JO - Macromolecular Rapid Communications

JF - Macromolecular Rapid Communications

SN - 1022-1336

IS - 23-24

ER -