Acquisition of HIV by african-born residents of Victoria, Australia

Insights from molecular epidemiology

Chris Lemoh, Claire E Ryan, Zamberi Sekawi, Anna C. Hearps, Eman Aleksic, Doris Chibo, Jeffrey Grierson, Samia Baho, Alan Street, Margaret Hellard, Beverley Ann Biggs, Suzanne M. Crowe

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

African-born Australians are a recognised "priority population" in Australia's Sixth National HIV/AIDS Strategy. We compared exposure location and route for African-born people living with HIV (PLHIV) in Victoria, Australia, with HIV-1 pol subtype from drug resistance assays and geographical origin suggested by phylogenetic analysis of env gene. Twenty adult HIV positive African-born Victorian residents were recruited via treating doctors. HIV exposure details were obtained from interviews and case notes. Viral RNA was extracted from participant stored plasma or whole blood. The env V3 region was sequenced and compared to globally representative reference HIV-1 sequences in the Los Alamos National Library HIV Database. Twelve participants reported exposure via heterosexual sex and two via iatrogenic blood exposures; four were men having sex with men (MSM); two were exposed via unknown routes. Eight participants reported exposure in their countries of birth, seven in Australia, three in other countries and two in unknown locations. Genotype results (pol) were available for ten participants. HIV env amplification was successful in eighteen cases. HIV-1 subtype was identified in all participants: eight both pol and env; ten env alone and two pol alone. Twelve were subtype C, four subtype B, three subtype A and one subtype CRF02-AG. Reported exposure location was consistent with the phylogenetic clustering of env sequences. African Australians are members of multiple transnational social and sexual networks influencing their exposure to HIV. Phylogenetic analysis may complement traditional surveillance to discern patterns of HIV exposure, providing focus for HIV prevention programs in mobile populations.

Original languageEnglish
Article numbere84008
Number of pages10
JournalPLoS ONE
Volume8
Issue number12
DOIs
Publication statusPublished - 31 Dec 2013
Externally publishedYes

Cite this

Lemoh, Chris ; Ryan, Claire E ; Sekawi, Zamberi ; Hearps, Anna C. ; Aleksic, Eman ; Chibo, Doris ; Grierson, Jeffrey ; Baho, Samia ; Street, Alan ; Hellard, Margaret ; Biggs, Beverley Ann ; Crowe, Suzanne M. / Acquisition of HIV by african-born residents of Victoria, Australia : Insights from molecular epidemiology. In: PLoS ONE. 2013 ; Vol. 8, No. 12.
@article{b68bea5f9beb4c0798f0a90c0f883f17,
title = "Acquisition of HIV by african-born residents of Victoria, Australia: Insights from molecular epidemiology",
abstract = "African-born Australians are a recognised {"}priority population{"} in Australia's Sixth National HIV/AIDS Strategy. We compared exposure location and route for African-born people living with HIV (PLHIV) in Victoria, Australia, with HIV-1 pol subtype from drug resistance assays and geographical origin suggested by phylogenetic analysis of env gene. Twenty adult HIV positive African-born Victorian residents were recruited via treating doctors. HIV exposure details were obtained from interviews and case notes. Viral RNA was extracted from participant stored plasma or whole blood. The env V3 region was sequenced and compared to globally representative reference HIV-1 sequences in the Los Alamos National Library HIV Database. Twelve participants reported exposure via heterosexual sex and two via iatrogenic blood exposures; four were men having sex with men (MSM); two were exposed via unknown routes. Eight participants reported exposure in their countries of birth, seven in Australia, three in other countries and two in unknown locations. Genotype results (pol) were available for ten participants. HIV env amplification was successful in eighteen cases. HIV-1 subtype was identified in all participants: eight both pol and env; ten env alone and two pol alone. Twelve were subtype C, four subtype B, three subtype A and one subtype CRF02-AG. Reported exposure location was consistent with the phylogenetic clustering of env sequences. African Australians are members of multiple transnational social and sexual networks influencing their exposure to HIV. Phylogenetic analysis may complement traditional surveillance to discern patterns of HIV exposure, providing focus for HIV prevention programs in mobile populations.",
author = "Chris Lemoh and Ryan, {Claire E} and Zamberi Sekawi and Hearps, {Anna C.} and Eman Aleksic and Doris Chibo and Jeffrey Grierson and Samia Baho and Alan Street and Margaret Hellard and Biggs, {Beverley Ann} and Crowe, {Suzanne M.}",
year = "2013",
month = "12",
day = "31",
doi = "10.1371/journal.pone.0084008",
language = "English",
volume = "8",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

Acquisition of HIV by african-born residents of Victoria, Australia : Insights from molecular epidemiology. / Lemoh, Chris; Ryan, Claire E; Sekawi, Zamberi; Hearps, Anna C.; Aleksic, Eman; Chibo, Doris; Grierson, Jeffrey; Baho, Samia; Street, Alan; Hellard, Margaret; Biggs, Beverley Ann; Crowe, Suzanne M.

In: PLoS ONE, Vol. 8, No. 12, e84008, 31.12.2013.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Acquisition of HIV by african-born residents of Victoria, Australia

T2 - Insights from molecular epidemiology

AU - Lemoh, Chris

AU - Ryan, Claire E

AU - Sekawi, Zamberi

AU - Hearps, Anna C.

AU - Aleksic, Eman

AU - Chibo, Doris

AU - Grierson, Jeffrey

AU - Baho, Samia

AU - Street, Alan

AU - Hellard, Margaret

AU - Biggs, Beverley Ann

AU - Crowe, Suzanne M.

PY - 2013/12/31

Y1 - 2013/12/31

N2 - African-born Australians are a recognised "priority population" in Australia's Sixth National HIV/AIDS Strategy. We compared exposure location and route for African-born people living with HIV (PLHIV) in Victoria, Australia, with HIV-1 pol subtype from drug resistance assays and geographical origin suggested by phylogenetic analysis of env gene. Twenty adult HIV positive African-born Victorian residents were recruited via treating doctors. HIV exposure details were obtained from interviews and case notes. Viral RNA was extracted from participant stored plasma or whole blood. The env V3 region was sequenced and compared to globally representative reference HIV-1 sequences in the Los Alamos National Library HIV Database. Twelve participants reported exposure via heterosexual sex and two via iatrogenic blood exposures; four were men having sex with men (MSM); two were exposed via unknown routes. Eight participants reported exposure in their countries of birth, seven in Australia, three in other countries and two in unknown locations. Genotype results (pol) were available for ten participants. HIV env amplification was successful in eighteen cases. HIV-1 subtype was identified in all participants: eight both pol and env; ten env alone and two pol alone. Twelve were subtype C, four subtype B, three subtype A and one subtype CRF02-AG. Reported exposure location was consistent with the phylogenetic clustering of env sequences. African Australians are members of multiple transnational social and sexual networks influencing their exposure to HIV. Phylogenetic analysis may complement traditional surveillance to discern patterns of HIV exposure, providing focus for HIV prevention programs in mobile populations.

AB - African-born Australians are a recognised "priority population" in Australia's Sixth National HIV/AIDS Strategy. We compared exposure location and route for African-born people living with HIV (PLHIV) in Victoria, Australia, with HIV-1 pol subtype from drug resistance assays and geographical origin suggested by phylogenetic analysis of env gene. Twenty adult HIV positive African-born Victorian residents were recruited via treating doctors. HIV exposure details were obtained from interviews and case notes. Viral RNA was extracted from participant stored plasma or whole blood. The env V3 region was sequenced and compared to globally representative reference HIV-1 sequences in the Los Alamos National Library HIV Database. Twelve participants reported exposure via heterosexual sex and two via iatrogenic blood exposures; four were men having sex with men (MSM); two were exposed via unknown routes. Eight participants reported exposure in their countries of birth, seven in Australia, three in other countries and two in unknown locations. Genotype results (pol) were available for ten participants. HIV env amplification was successful in eighteen cases. HIV-1 subtype was identified in all participants: eight both pol and env; ten env alone and two pol alone. Twelve were subtype C, four subtype B, three subtype A and one subtype CRF02-AG. Reported exposure location was consistent with the phylogenetic clustering of env sequences. African Australians are members of multiple transnational social and sexual networks influencing their exposure to HIV. Phylogenetic analysis may complement traditional surveillance to discern patterns of HIV exposure, providing focus for HIV prevention programs in mobile populations.

UR - http://www.scopus.com/inward/record.url?scp=84894196314&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0084008

DO - 10.1371/journal.pone.0084008

M3 - Article

VL - 8

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 12

M1 - e84008

ER -