Acetylcholine induces oscillations in intracellular calcium in isolated bovine adrenal zona fasciculata/reticularis cells

Colin D. Clyne, Ian M. Bird, Simon W. Walker, Brent C. Williams

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2 Citations (Scopus)

Abstract

The effects of acetylcholine (ACh) on intracellular free calcium were studied in primary cultures of purified bovine adrenal zona fasciculata/reticularis (ZFR) cells. In fura-2 loaded single cells, concentrations of ACh which stimulated cortisol secretion and phosphoinositol production were found to promote an increase in free cytosolic calcium ([Ca2+]i). This response was heterogeneous, showing either (i) an initial increase in [Ca2+]i followed by a fall to a level above that in unstimulated cells, or (ii) an initial increase followed by oscillations about the original resting level or a higher resting level. The frequencies of [Ca2+]i oscillations to ACh showed a dose-dependent trend. The sustained [Ca2+]i oscillations were abolished by the muscarinic antagonist atropine, or by removal of extracellular Ca2+. These data demonstrate for the first time in adrenocortical cells that: (i) ACh can induce [Ca2+]i oscillations in single ZFR cells; (ii) these oscillations occur in a dose-dependent manner; (iii) the sustained oscillatory phase is dependent on influx of extracellular Ca2+. Thus, like cells of the zona glomerulosa, bovine ZFR cells are also capable of sustained dose-dependent oscillatory responses to agonists which activate phosphoinositidase C.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalEndocrine Research
Volume21
Issue number1-2
DOIs
Publication statusPublished - 1 Jan 1995
Externally publishedYes

Cite this

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abstract = "The effects of acetylcholine (ACh) on intracellular free calcium were studied in primary cultures of purified bovine adrenal zona fasciculata/reticularis (ZFR) cells. In fura-2 loaded single cells, concentrations of ACh which stimulated cortisol secretion and phosphoinositol production were found to promote an increase in free cytosolic calcium ([Ca2+]i). This response was heterogeneous, showing either (i) an initial increase in [Ca2+]i followed by a fall to a level above that in unstimulated cells, or (ii) an initial increase followed by oscillations about the original resting level or a higher resting level. The frequencies of [Ca2+]i oscillations to ACh showed a dose-dependent trend. The sustained [Ca2+]i oscillations were abolished by the muscarinic antagonist atropine, or by removal of extracellular Ca2+. These data demonstrate for the first time in adrenocortical cells that: (i) ACh can induce [Ca2+]i oscillations in single ZFR cells; (ii) these oscillations occur in a dose-dependent manner; (iii) the sustained oscillatory phase is dependent on influx of extracellular Ca2+. Thus, like cells of the zona glomerulosa, bovine ZFR cells are also capable of sustained dose-dependent oscillatory responses to agonists which activate phosphoinositidase C.",
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Acetylcholine induces oscillations in intracellular calcium in isolated bovine adrenal zona fasciculata/reticularis cells. / Clyne, Colin D.; Bird, Ian M.; Walker, Simon W.; Williams, Brent C.

In: Endocrine Research, Vol. 21, No. 1-2, 01.01.1995, p. 53-60.

Research output: Contribution to journalArticleResearchpeer-review

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