Accentuated antagonism of vagal heart rate control and less potent prejunctional inhibition of vagal acetylcholine release during sympathetic nerve stimulation in the rat

Toru Kawada, Takashi Sonobe, Yohsuke Hayama, Takuya Nishikawa, Tadayoshi Miyamoto, Tsuyoshi Akiyama, James T. Pearson, Masaru Sugimachi

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Complex interactions are known to occur between the sympathetic and parasympathetic controls of the heart. Although sympathetic nerve stimulation (SNS) usually augments the heart rate (HR) response to vagal nerve stimulation (VNS), exogenously administered norepinephrine (NE) can attenuate the HR response as well as the myocardial interstitial acetylcholine (ACh) release during VNS. To provide a basis for an integrative knowledge about the opposing adrenergic effects on the vagal control of the heart, we examined whether SNS significantly attenuates VNS-induced myocardial interstitial ACh release in the in vivo beating heart. In nine anesthetized rats, changes in HR and myocardial interstitial ACh release in response to 5- and 20-Hz VNS were examined in both the absence and presence of a 5-Hz background SNS. The SNS significantly enhanced the VNS-induced HR reduction during 20-Hz VNS (−101.2 ± 33.1 vs. –163.0 ± 34.9 beats/min, P < 0.001, a 60% augmentation). By contrast, the SNS significantly attenuated the ACh release during 20-Hz VNS (4.30 ± 0.72 vs. 3.80 ± 0.75 nM, P < 0.01, a 12% attenuation). In conclusion, SNS exerted only a moderate inhibitory effect on the VNS-induced myocardial interstitial ACh release in the in vivo beating heart.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalAutonomic Neuroscience: Basic and Clinical
Volume218
DOIs
Publication statusPublished - 1 May 2019
Externally publishedYes

Keywords

  • Acetylcholine
  • Heart rate
  • Rats
  • Sympathetic nerve stimulation
  • Vagal nerve stimulation

Cite this

Kawada, Toru ; Sonobe, Takashi ; Hayama, Yohsuke ; Nishikawa, Takuya ; Miyamoto, Tadayoshi ; Akiyama, Tsuyoshi ; Pearson, James T. ; Sugimachi, Masaru. / Accentuated antagonism of vagal heart rate control and less potent prejunctional inhibition of vagal acetylcholine release during sympathetic nerve stimulation in the rat. In: Autonomic Neuroscience: Basic and Clinical. 2019 ; Vol. 218. pp. 25-30.
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abstract = "Complex interactions are known to occur between the sympathetic and parasympathetic controls of the heart. Although sympathetic nerve stimulation (SNS) usually augments the heart rate (HR) response to vagal nerve stimulation (VNS), exogenously administered norepinephrine (NE) can attenuate the HR response as well as the myocardial interstitial acetylcholine (ACh) release during VNS. To provide a basis for an integrative knowledge about the opposing adrenergic effects on the vagal control of the heart, we examined whether SNS significantly attenuates VNS-induced myocardial interstitial ACh release in the in vivo beating heart. In nine anesthetized rats, changes in HR and myocardial interstitial ACh release in response to 5- and 20-Hz VNS were examined in both the absence and presence of a 5-Hz background SNS. The SNS significantly enhanced the VNS-induced HR reduction during 20-Hz VNS (−101.2 ± 33.1 vs. –163.0 ± 34.9 beats/min, P < 0.001, a 60{\%} augmentation). By contrast, the SNS significantly attenuated the ACh release during 20-Hz VNS (4.30 ± 0.72 vs. 3.80 ± 0.75 nM, P < 0.01, a 12{\%} attenuation). In conclusion, SNS exerted only a moderate inhibitory effect on the VNS-induced myocardial interstitial ACh release in the in vivo beating heart.",
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Accentuated antagonism of vagal heart rate control and less potent prejunctional inhibition of vagal acetylcholine release during sympathetic nerve stimulation in the rat. / Kawada, Toru; Sonobe, Takashi; Hayama, Yohsuke; Nishikawa, Takuya; Miyamoto, Tadayoshi; Akiyama, Tsuyoshi; Pearson, James T.; Sugimachi, Masaru.

In: Autonomic Neuroscience: Basic and Clinical, Vol. 218, 01.05.2019, p. 25-30.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Kawada, Toru

AU - Sonobe, Takashi

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