Accelerating Chemobehavioral Phenotypic Screening in Neurotoxicology Using a Living Embryo Array System

Jason Henry, Yutao Bai, Florian Kreuder, David Mawdsley, Jan Kaslin, Donald Wlodkowic

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Large-scale chemobehavioral phenotyping with zebrafish embryos is a promising avenue for accelerated neurotoxicity testing and discovery of behavior-modifying neuroceuticals. These strategies are hampered by lack of effective embryo in-test positioning, wide-field imaging, and high-throughput bioinformatic analytics. In this study, we demonstrate advantages of using custom large-density embryo arrays in conjunction with an open-source ultra-high-definition video imaging system. Moreover, we present a high-throughput bioinformatics workflow for rapid behavioral analysis of large cohorts of specimens in photomotor response bioassays. The system validation was showcased in a proof-of-concept neurotoxicity analysis.

Original languageEnglish
Pages (from-to)32-35
Number of pages4
JournalZebrafish
Volume19
Issue number1
DOIs
Publication statusPublished - Feb 2022

Keywords

  • array
  • automated
  • behavior
  • ecotoxicology
  • embryo
  • neurotoxicity
  • screening
  • zebrafish

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