Accelerated clearance of Escherichia coli in experimental peritonitis of histamine-deficient mice

Yoshio Hori, Yoshihiro Nihei, Yoshimochi Kurokawa, Atsuo Kuramasu, Yoko Makabe-Kobayashi, Tadashi Terui, Hideyuki Doi, Susumu Satomi, Eiko Sakurai, Andras Nagy, Takehiko Watanabe, Hiroshi Ohtsu

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Abstract

We prepared a model of experimental peritonitis by introducing Escherichia coli into the peritoneal cavity of the histamine-deficient mice generated by a disruption of the gene for histidine decarboxylase (HDC), the unique histamine-synthesizing enzyme. When we inoculated E. coli into the peritoneal cavities of the HDC-/- (histamine-deficient) mice, they eliminated E. coli more efficiently than did the wild-type mice. Histamine was released efficiently from the peritoneal cells after E. coli inoculation in HDC+/+ mice, although only trace amounts were detected in the peritoneal cells of HDC-/- mice. Two histamine agonists (6-[2-(4-imidazolyl)ethylamino]-N-(4-trifluoromethylphenyl) hepatanecarboxamide (H1) and dimaprit (H2)) impaired the clearance of E. coli from the peritoneal cavity in HDC-/- mice, suggesting that the activation of both H1 and H2 receptors suppresses the clearance. In contrast, two kinds of H1 and H2 receptor antagonists, cimetidine and pyrilamine, promoted the clearance of E. coli in HDC+/+ mice. Phagocytosis appeared to be enhanced in HDC-/- mice, since the number of neutrophils in the peritoneal cavity of HDC-/- mice was markedly increased. This enhanced recruitment of neutrophils was suppressed in the presence of the histamine agonists, 6-[2-(4-imidazolyl)ethylamino]-N-(4-trifluoromethylphenyl)hepatanecarboxamide and dimaprit. In this report histamine was first shown to be an important mediator in an E. coli infectious peritonitis model, causing a delay in the elimination of bacteria. This also raised the possibility of the use of antihistamine drugs for bacterial infection.

Original languageEnglish
Pages (from-to)1978-1983
Number of pages6
JournalJournal of Immunology
Volume169
Issue number4
DOIs
Publication statusPublished - 15 Aug 2002
Externally publishedYes

Cite this

Hori, Y., Nihei, Y., Kurokawa, Y., Kuramasu, A., Makabe-Kobayashi, Y., Terui, T., ... Ohtsu, H. (2002). Accelerated clearance of Escherichia coli in experimental peritonitis of histamine-deficient mice. Journal of Immunology, 169(4), 1978-1983. https://doi.org/10.4049/jimmunol.169.4.1978
Hori, Yoshio ; Nihei, Yoshihiro ; Kurokawa, Yoshimochi ; Kuramasu, Atsuo ; Makabe-Kobayashi, Yoko ; Terui, Tadashi ; Doi, Hideyuki ; Satomi, Susumu ; Sakurai, Eiko ; Nagy, Andras ; Watanabe, Takehiko ; Ohtsu, Hiroshi. / Accelerated clearance of Escherichia coli in experimental peritonitis of histamine-deficient mice. In: Journal of Immunology. 2002 ; Vol. 169, No. 4. pp. 1978-1983.
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abstract = "We prepared a model of experimental peritonitis by introducing Escherichia coli into the peritoneal cavity of the histamine-deficient mice generated by a disruption of the gene for histidine decarboxylase (HDC), the unique histamine-synthesizing enzyme. When we inoculated E. coli into the peritoneal cavities of the HDC-/- (histamine-deficient) mice, they eliminated E. coli more efficiently than did the wild-type mice. Histamine was released efficiently from the peritoneal cells after E. coli inoculation in HDC+/+ mice, although only trace amounts were detected in the peritoneal cells of HDC-/- mice. Two histamine agonists (6-[2-(4-imidazolyl)ethylamino]-N-(4-trifluoromethylphenyl) hepatanecarboxamide (H1) and dimaprit (H2)) impaired the clearance of E. coli from the peritoneal cavity in HDC-/- mice, suggesting that the activation of both H1 and H2 receptors suppresses the clearance. In contrast, two kinds of H1 and H2 receptor antagonists, cimetidine and pyrilamine, promoted the clearance of E. coli in HDC+/+ mice. Phagocytosis appeared to be enhanced in HDC-/- mice, since the number of neutrophils in the peritoneal cavity of HDC-/- mice was markedly increased. This enhanced recruitment of neutrophils was suppressed in the presence of the histamine agonists, 6-[2-(4-imidazolyl)ethylamino]-N-(4-trifluoromethylphenyl)hepatanecarboxamide and dimaprit. In this report histamine was first shown to be an important mediator in an E. coli infectious peritonitis model, causing a delay in the elimination of bacteria. This also raised the possibility of the use of antihistamine drugs for bacterial infection.",
author = "Yoshio Hori and Yoshihiro Nihei and Yoshimochi Kurokawa and Atsuo Kuramasu and Yoko Makabe-Kobayashi and Tadashi Terui and Hideyuki Doi and Susumu Satomi and Eiko Sakurai and Andras Nagy and Takehiko Watanabe and Hiroshi Ohtsu",
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Hori, Y, Nihei, Y, Kurokawa, Y, Kuramasu, A, Makabe-Kobayashi, Y, Terui, T, Doi, H, Satomi, S, Sakurai, E, Nagy, A, Watanabe, T & Ohtsu, H 2002, 'Accelerated clearance of Escherichia coli in experimental peritonitis of histamine-deficient mice' Journal of Immunology, vol. 169, no. 4, pp. 1978-1983. https://doi.org/10.4049/jimmunol.169.4.1978

Accelerated clearance of Escherichia coli in experimental peritonitis of histamine-deficient mice. / Hori, Yoshio; Nihei, Yoshihiro; Kurokawa, Yoshimochi; Kuramasu, Atsuo; Makabe-Kobayashi, Yoko; Terui, Tadashi; Doi, Hideyuki; Satomi, Susumu; Sakurai, Eiko; Nagy, Andras; Watanabe, Takehiko; Ohtsu, Hiroshi.

In: Journal of Immunology, Vol. 169, No. 4, 15.08.2002, p. 1978-1983.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Accelerated clearance of Escherichia coli in experimental peritonitis of histamine-deficient mice

AU - Hori, Yoshio

AU - Nihei, Yoshihiro

AU - Kurokawa, Yoshimochi

AU - Kuramasu, Atsuo

AU - Makabe-Kobayashi, Yoko

AU - Terui, Tadashi

AU - Doi, Hideyuki

AU - Satomi, Susumu

AU - Sakurai, Eiko

AU - Nagy, Andras

AU - Watanabe, Takehiko

AU - Ohtsu, Hiroshi

PY - 2002/8/15

Y1 - 2002/8/15

N2 - We prepared a model of experimental peritonitis by introducing Escherichia coli into the peritoneal cavity of the histamine-deficient mice generated by a disruption of the gene for histidine decarboxylase (HDC), the unique histamine-synthesizing enzyme. When we inoculated E. coli into the peritoneal cavities of the HDC-/- (histamine-deficient) mice, they eliminated E. coli more efficiently than did the wild-type mice. Histamine was released efficiently from the peritoneal cells after E. coli inoculation in HDC+/+ mice, although only trace amounts were detected in the peritoneal cells of HDC-/- mice. Two histamine agonists (6-[2-(4-imidazolyl)ethylamino]-N-(4-trifluoromethylphenyl) hepatanecarboxamide (H1) and dimaprit (H2)) impaired the clearance of E. coli from the peritoneal cavity in HDC-/- mice, suggesting that the activation of both H1 and H2 receptors suppresses the clearance. In contrast, two kinds of H1 and H2 receptor antagonists, cimetidine and pyrilamine, promoted the clearance of E. coli in HDC+/+ mice. Phagocytosis appeared to be enhanced in HDC-/- mice, since the number of neutrophils in the peritoneal cavity of HDC-/- mice was markedly increased. This enhanced recruitment of neutrophils was suppressed in the presence of the histamine agonists, 6-[2-(4-imidazolyl)ethylamino]-N-(4-trifluoromethylphenyl)hepatanecarboxamide and dimaprit. In this report histamine was first shown to be an important mediator in an E. coli infectious peritonitis model, causing a delay in the elimination of bacteria. This also raised the possibility of the use of antihistamine drugs for bacterial infection.

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Hori Y, Nihei Y, Kurokawa Y, Kuramasu A, Makabe-Kobayashi Y, Terui T et al. Accelerated clearance of Escherichia coli in experimental peritonitis of histamine-deficient mice. Journal of Immunology. 2002 Aug 15;169(4):1978-1983. https://doi.org/10.4049/jimmunol.169.4.1978