Abundant calcitonin receptors in isolated rat osteoclasts. Biochemical and autoradiographic characterization

G. C. Nicholson, J. M. Moseley, P. M. Sexton, F. A. Mendelsohn, T. J. Martin

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Abstract

Calcitonin receptors have been characterized for the first time in isolated osteoclasts. These receptors have been demonstrated by autoradiographic and biochemical methods, and the cells have also been shown to respond to calcitonin with a dose-dependent increase in cyclic AMP. The receptors in rat osteoclasts are specific and of high affinity (dissociation constant, K(d), 1 to 6 x 10 -10 M), and are present in greater numbers than in any cell previously studied (>10 6 per cell). Chemical cross-linking of 125 I-labeled salmon calcitonin to osteoclasts using disuccinimidyl suberate resulted in identification of a receptor component with a relative molecular weight of 80,000-90,000. By counting grains in autoradiographic experiments, we found that >80% of specifically bound radioactivity was associated with multinucleate osteoclasts and the remainder was associated with mononuclear cells that are not osteoblasts, but that may be osteoclast precursors.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalJournal of Clinical Investigation
Volume78
Issue number2
DOIs
Publication statusPublished - 1 Jan 1986
Externally publishedYes

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