TY - JOUR
T1 - Absence of surface IgD does not impair naive B cell homeostasis or memory B cell formation in IGHD haploinsufficient humans
AU - Nechvatalova, Jana
AU - Bartol, Sophinus J.W.
AU - Chovancova, Zita
AU - Boon, Louis
AU - Vlkova, Marcela
AU - Van Zelm, Menno C.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Surface IgD is coexpressed with IgM on naive mature B cells. Still, the role of surface IgD remains enigmatic even 50 y after its initial discovery. In this study, we examined the in vivo role of surface IgD in human B cell homeostasis and Ab responses in four individuals with heterozygous nonsense mutations in IGHD. All IGHD heterozygous individuals had normal numbers of B cells and serum Igs and did not show signs of immunodeficiency or immune dysregulation. IgD+ and IgD2 naive mature B cells were present in equal numbers and showed similar immunophenotypes, except for decreased expression of CD79b in the IgD2 subset. Furthermore, both IgD+ and IgD2 naive mature B cells had normal replication histories and similar capacities to differentiate into plasma cells upon in vitro stimulation, and Ig class-switched memory B cells showed similar levels of somatic hypermutations. Thus, human B cells lacking IgD expression develop normally and generate immunological memory in vivo, suggesting that surface IgD might function more restrictedly in regulating of B cell activation to specific antigenic structures.
AB - Surface IgD is coexpressed with IgM on naive mature B cells. Still, the role of surface IgD remains enigmatic even 50 y after its initial discovery. In this study, we examined the in vivo role of surface IgD in human B cell homeostasis and Ab responses in four individuals with heterozygous nonsense mutations in IGHD. All IGHD heterozygous individuals had normal numbers of B cells and serum Igs and did not show signs of immunodeficiency or immune dysregulation. IgD+ and IgD2 naive mature B cells were present in equal numbers and showed similar immunophenotypes, except for decreased expression of CD79b in the IgD2 subset. Furthermore, both IgD+ and IgD2 naive mature B cells had normal replication histories and similar capacities to differentiate into plasma cells upon in vitro stimulation, and Ig class-switched memory B cells showed similar levels of somatic hypermutations. Thus, human B cells lacking IgD expression develop normally and generate immunological memory in vivo, suggesting that surface IgD might function more restrictedly in regulating of B cell activation to specific antigenic structures.
UR - http://www.scopus.com/inward/record.url?scp=85053483712&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.1800767
DO - 10.4049/jimmunol.1800767
M3 - Article
AN - SCOPUS:85053483712
VL - 201
SP - 1928
EP - 1935
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 7
ER -