Absence of surface IgD does not impair naive B cell homeostasis or memory B cell formation in IGHD haploinsufficient humans

Jana Nechvatalova, Sophinus J.W. Bartol, Zita Chovancova, Louis Boon, Marcela Vlkova, Menno C. Van Zelm

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Abstract

Surface IgD is coexpressed with IgM on naive mature B cells. Still, the role of surface IgD remains enigmatic even 50 y after its initial discovery. In this study, we examined the in vivo role of surface IgD in human B cell homeostasis and Ab responses in four individuals with heterozygous nonsense mutations in IGHD. All IGHD heterozygous individuals had normal numbers of B cells and serum Igs and did not show signs of immunodeficiency or immune dysregulation. IgD+ and IgD2 naive mature B cells were present in equal numbers and showed similar immunophenotypes, except for decreased expression of CD79b in the IgD2 subset. Furthermore, both IgD+ and IgD2 naive mature B cells had normal replication histories and similar capacities to differentiate into plasma cells upon in vitro stimulation, and Ig class-switched memory B cells showed similar levels of somatic hypermutations. Thus, human B cells lacking IgD expression develop normally and generate immunological memory in vivo, suggesting that surface IgD might function more restrictedly in regulating of B cell activation to specific antigenic structures.

Original languageEnglish
Pages (from-to)1928-1935
Number of pages8
JournalJournal of Immunology
Volume201
Issue number7
DOIs
Publication statusPublished - 1 Oct 2018

Cite this

Nechvatalova, Jana ; Bartol, Sophinus J.W. ; Chovancova, Zita ; Boon, Louis ; Vlkova, Marcela ; Van Zelm, Menno C. / Absence of surface IgD does not impair naive B cell homeostasis or memory B cell formation in IGHD haploinsufficient humans. In: Journal of Immunology. 2018 ; Vol. 201, No. 7. pp. 1928-1935.
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abstract = "Surface IgD is coexpressed with IgM on naive mature B cells. Still, the role of surface IgD remains enigmatic even 50 y after its initial discovery. In this study, we examined the in vivo role of surface IgD in human B cell homeostasis and Ab responses in four individuals with heterozygous nonsense mutations in IGHD. All IGHD heterozygous individuals had normal numbers of B cells and serum Igs and did not show signs of immunodeficiency or immune dysregulation. IgD+ and IgD2 naive mature B cells were present in equal numbers and showed similar immunophenotypes, except for decreased expression of CD79b in the IgD2 subset. Furthermore, both IgD+ and IgD2 naive mature B cells had normal replication histories and similar capacities to differentiate into plasma cells upon in vitro stimulation, and Ig class-switched memory B cells showed similar levels of somatic hypermutations. Thus, human B cells lacking IgD expression develop normally and generate immunological memory in vivo, suggesting that surface IgD might function more restrictedly in regulating of B cell activation to specific antigenic structures.",
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Absence of surface IgD does not impair naive B cell homeostasis or memory B cell formation in IGHD haploinsufficient humans. / Nechvatalova, Jana; Bartol, Sophinus J.W.; Chovancova, Zita; Boon, Louis; Vlkova, Marcela; Van Zelm, Menno C.

In: Journal of Immunology, Vol. 201, No. 7, 01.10.2018, p. 1928-1935.

Research output: Contribution to journalArticleResearchpeer-review

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AB - Surface IgD is coexpressed with IgM on naive mature B cells. Still, the role of surface IgD remains enigmatic even 50 y after its initial discovery. In this study, we examined the in vivo role of surface IgD in human B cell homeostasis and Ab responses in four individuals with heterozygous nonsense mutations in IGHD. All IGHD heterozygous individuals had normal numbers of B cells and serum Igs and did not show signs of immunodeficiency or immune dysregulation. IgD+ and IgD2 naive mature B cells were present in equal numbers and showed similar immunophenotypes, except for decreased expression of CD79b in the IgD2 subset. Furthermore, both IgD+ and IgD2 naive mature B cells had normal replication histories and similar capacities to differentiate into plasma cells upon in vitro stimulation, and Ig class-switched memory B cells showed similar levels of somatic hypermutations. Thus, human B cells lacking IgD expression develop normally and generate immunological memory in vivo, suggesting that surface IgD might function more restrictedly in regulating of B cell activation to specific antigenic structures.

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