ABO genotyping-identification of O1, O(1*), and O2 alleles using the polymerase chain reaction - Sequence specific oligonucleotide (PCR-SSO) technique

N. A. Mifsud, A. P. Haddad, J. A. Condon, R. L. Sparrow

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Abstract

ABO polymorphism at the gene level has been investigated by molecular methods, predominantly sequencing and restriction fragment length polymorphism (RFLP). We describe the application of the polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) method, which is considered to be more versatile for large sample numbers, compared with conventional ABO genotyping by PCR-RFLP. PCR-SSO, while maintaing accurate andd reliable results, reduces costs and labor. A population of 155 random individuals was investigated for the three O alleles, O1, O(1*), and O2. The allelic frequencies were 35 percent, 26 percent, and 5 percent, respectively. PCR-SSO results correlated completely with both serologic and PCR-RFLP results.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalImmunohematology
Volume12
Issue number4
Publication statusPublished - 1996
Externally publishedYes

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