Abnormalities in CD57+ cytotoxic T cells and Vδ1+ γδT cells in subclinical celiac disease in childhood are affected by cytomegalovirus. The Generation R Study

Michelle A E Jansen, Diana Van Den Heuvel, Vincent W V Jaddoe, M. C. van Zelm, Henriëtte A. Moll

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Celiac disease (CD) is a digestive and autoimmune disorder driven by an immune response to modified gluten peptides. Affected intestines show infiltrates of various T-cell and NK-cell subsets. It is currently unclear if individuals with subclinical CD have systemic abnormalities in immune cells. We here studied whether subclinical CD is associated with changes in blood CD57-expressing and Vδ1-expressing lymphocytes in children, and whether cytomegalovirus (CMV) infection modifies this association. Included were 1068 children from the Generation R Study. Serum Immunoglobulin G (IgG) levels against CMV were measured by ELISA; Tissue transglutaminase type 2 antibody (TG2A) levels with fluorescence enzyme immunoassay (FEIA). Duodenal biopsies, additional Human Leukocyte Antigen (HLA) DQ 2.2, 2.5 and 8 and endomysial antibody (EMA) typing were performed in TG2A positive children. Subclinical CD cases (n = 12) had 1.8 fold (95% CI 1.06; 3.1) fewer Vδ1. + T cells which was predominantly observed in CMV seronegative children (p-interaction 0.02), and 2.7 fold (95% CI 1.25; 5.99) more CD57. + T cells than HLA DQ2/-DQ8 positive controls (n = 339). Hence, children with subclinical CD have alterations in specific blood T cell subsets that are linked to viral pathology. The observed interaction effect between subclinical CD and CMV may contribute to the understanding of disease pathogenesis.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalClinical Immunology
Volume183
DOIs
Publication statusPublished - Oct 2017

Keywords

  • CD57+ T cells
  • Celiac disease
  • Child
  • Cohort study
  • TG2A levels
  • Vδ1+ T cells

Cite this

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title = "Abnormalities in CD57+ cytotoxic T cells and Vδ1+ γδT cells in subclinical celiac disease in childhood are affected by cytomegalovirus. The Generation R Study",
abstract = "Celiac disease (CD) is a digestive and autoimmune disorder driven by an immune response to modified gluten peptides. Affected intestines show infiltrates of various T-cell and NK-cell subsets. It is currently unclear if individuals with subclinical CD have systemic abnormalities in immune cells. We here studied whether subclinical CD is associated with changes in blood CD57-expressing and Vδ1-expressing lymphocytes in children, and whether cytomegalovirus (CMV) infection modifies this association. Included were 1068 children from the Generation R Study. Serum Immunoglobulin G (IgG) levels against CMV were measured by ELISA; Tissue transglutaminase type 2 antibody (TG2A) levels with fluorescence enzyme immunoassay (FEIA). Duodenal biopsies, additional Human Leukocyte Antigen (HLA) DQ 2.2, 2.5 and 8 and endomysial antibody (EMA) typing were performed in TG2A positive children. Subclinical CD cases (n = 12) had 1.8 fold (95{\%} CI 1.06; 3.1) fewer Vδ1. + T cells which was predominantly observed in CMV seronegative children (p-interaction 0.02), and 2.7 fold (95{\%} CI 1.25; 5.99) more CD57. + T cells than HLA DQ2/-DQ8 positive controls (n = 339). Hence, children with subclinical CD have alterations in specific blood T cell subsets that are linked to viral pathology. The observed interaction effect between subclinical CD and CMV may contribute to the understanding of disease pathogenesis.",
keywords = "CD57+ T cells, Celiac disease, Child, Cohort study, TG2A levels, Vδ1+ T cells",
author = "Jansen, {Michelle A E} and {Van Den Heuvel}, Diana and Jaddoe, {Vincent W V} and {van Zelm}, {M. C.} and Moll, {Henri{\"e}tte A.}",
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Abnormalities in CD57+ cytotoxic T cells and Vδ1+ γδT cells in subclinical celiac disease in childhood are affected by cytomegalovirus. The Generation R Study. / Jansen, Michelle A E; Van Den Heuvel, Diana; Jaddoe, Vincent W V; van Zelm, M. C.; Moll, Henriëtte A.

In: Clinical Immunology, Vol. 183, 10.2017, p. 233-239.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Jansen, Michelle A E

AU - Van Den Heuvel, Diana

AU - Jaddoe, Vincent W V

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AU - Moll, Henriëtte A.

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