Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy

Eleni Giannakis, Chrishan Samuel, Tim D Hewitson, Wee Ming Boon, Mary Macris, Shane Bernadine Reeve, Josie Michelle Lawrence, Alexander Ian Smith, Geoffrey W Tregear, Johh D Wade

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Kidney failure is a major health problem worldwide. Patients with end-stage renal disease require intensive medical support by dialysis or kidney transplantation. Current methods for diagnosis of kidney disease are either invasive or insensitive, and renal function may decline by as much as 50 before it can be detected using current techniques. The goal of this study was, therefore, to identify biomarkers of kidney disease (associated with renal fibrosis) that can be used for the development of a non-invasive clinical test for early disease detection. We utilized two protein-profiling technologies (SELDI-TOF MS and 2-D) to screen the plasma and kidney proteome for aberrantly expressed proteins in an experimental mouse model of unilateral uretric obstruction, which mimics the pathology of human renal disease. Several differentially regulated proteins were detected at the plasma level of day-3-obstructed animals, which included serum amyloid A1, fibrinogen ?, haptoglobin precursor protein, haptoglobin and major urinary proteins 11 and 8. Differentially expressed proteins detected at the tissue level included ras-like activator protein 2, haptoglobin precursor protein, malate dehydrogenase, ? enolase and murine urinary protein (all p
Original languageEnglish
Pages (from-to)1211 - 1224
Number of pages14
JournalProteomics - Clinical Applications
Volume3
Issue number10
DOIs
Publication statusPublished - 2009

Cite this

Giannakis, Eleni ; Samuel, Chrishan ; Hewitson, Tim D ; Boon, Wee Ming ; Macris, Mary ; Reeve, Shane Bernadine ; Lawrence, Josie Michelle ; Smith, Alexander Ian ; Tregear, Geoffrey W ; Wade, Johh D. / Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy. In: Proteomics - Clinical Applications. 2009 ; Vol. 3, No. 10. pp. 1211 - 1224.
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title = "Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy",
abstract = "Kidney failure is a major health problem worldwide. Patients with end-stage renal disease require intensive medical support by dialysis or kidney transplantation. Current methods for diagnosis of kidney disease are either invasive or insensitive, and renal function may decline by as much as 50 before it can be detected using current techniques. The goal of this study was, therefore, to identify biomarkers of kidney disease (associated with renal fibrosis) that can be used for the development of a non-invasive clinical test for early disease detection. We utilized two protein-profiling technologies (SELDI-TOF MS and 2-D) to screen the plasma and kidney proteome for aberrantly expressed proteins in an experimental mouse model of unilateral uretric obstruction, which mimics the pathology of human renal disease. Several differentially regulated proteins were detected at the plasma level of day-3-obstructed animals, which included serum amyloid A1, fibrinogen ?, haptoglobin precursor protein, haptoglobin and major urinary proteins 11 and 8. Differentially expressed proteins detected at the tissue level included ras-like activator protein 2, haptoglobin precursor protein, malate dehydrogenase, ? enolase and murine urinary protein (all p",
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Giannakis, E, Samuel, C, Hewitson, TD, Boon, WM, Macris, M, Reeve, SB, Lawrence, JM, Smith, AI, Tregear, GW & Wade, JD 2009, 'Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy', Proteomics - Clinical Applications, vol. 3, no. 10, pp. 1211 - 1224. https://doi.org/10.1002/prca.200900021

Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy. / Giannakis, Eleni; Samuel, Chrishan; Hewitson, Tim D; Boon, Wee Ming; Macris, Mary; Reeve, Shane Bernadine; Lawrence, Josie Michelle; Smith, Alexander Ian; Tregear, Geoffrey W; Wade, Johh D.

In: Proteomics - Clinical Applications, Vol. 3, No. 10, 2009, p. 1211 - 1224.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy

AU - Giannakis, Eleni

AU - Samuel, Chrishan

AU - Hewitson, Tim D

AU - Boon, Wee Ming

AU - Macris, Mary

AU - Reeve, Shane Bernadine

AU - Lawrence, Josie Michelle

AU - Smith, Alexander Ian

AU - Tregear, Geoffrey W

AU - Wade, Johh D

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