TY - JOUR
T1 - Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy
AU - Giannakis, Eleni
AU - Samuel, Chrishan
AU - Hewitson, Tim D
AU - Boon, Wee Ming
AU - Macris, Mary
AU - Reeve, Shane Bernadine
AU - Lawrence, Josie Michelle
AU - Smith, Alexander Ian
AU - Tregear, Geoffrey W
AU - Wade, Johh D
PY - 2009
Y1 - 2009
N2 - Kidney failure is a major health problem worldwide. Patients with end-stage renal disease require intensive medical support by dialysis or kidney transplantation. Current methods for diagnosis of kidney disease are either invasive or insensitive, and renal function may decline by as much as 50 before it can be detected using current techniques. The goal of this study was, therefore, to identify biomarkers of kidney disease (associated with renal fibrosis) that can be used for the development of a non-invasive clinical test for early disease detection. We utilized two protein-profiling technologies (SELDI-TOF MS and 2-D) to screen the plasma and kidney proteome for aberrantly expressed proteins in an experimental mouse model of unilateral uretric obstruction, which mimics the pathology of human renal disease. Several differentially regulated proteins were detected at the plasma level of day-3-obstructed animals, which included serum amyloid A1, fibrinogen ?, haptoglobin precursor protein, haptoglobin and major urinary proteins 11 and 8. Differentially expressed proteins detected at the tissue level included ras-like activator protein 2, haptoglobin precursor protein, malate dehydrogenase, ? enolase and murine urinary protein (all p
AB - Kidney failure is a major health problem worldwide. Patients with end-stage renal disease require intensive medical support by dialysis or kidney transplantation. Current methods for diagnosis of kidney disease are either invasive or insensitive, and renal function may decline by as much as 50 before it can be detected using current techniques. The goal of this study was, therefore, to identify biomarkers of kidney disease (associated with renal fibrosis) that can be used for the development of a non-invasive clinical test for early disease detection. We utilized two protein-profiling technologies (SELDI-TOF MS and 2-D) to screen the plasma and kidney proteome for aberrantly expressed proteins in an experimental mouse model of unilateral uretric obstruction, which mimics the pathology of human renal disease. Several differentially regulated proteins were detected at the plasma level of day-3-obstructed animals, which included serum amyloid A1, fibrinogen ?, haptoglobin precursor protein, haptoglobin and major urinary proteins 11 and 8. Differentially expressed proteins detected at the tissue level included ras-like activator protein 2, haptoglobin precursor protein, malate dehydrogenase, ? enolase and murine urinary protein (all p
UR - http://www3.interscience.wiley.com/cgi-bin/fulltext/122534859/PDFSTART
U2 - 10.1002/prca.200900021
DO - 10.1002/prca.200900021
M3 - Article
SN - 1862-8346
VL - 3
SP - 1211
EP - 1224
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
IS - 10
ER -