ABCA12 regulates insulin secretion from β-cells

Gloria M. Ursino, Ying Fu, Denny L. Cottle, Nigora Mukhamedova, Lynelle K. Jones, Hann Low, Ming Shen Tham, Wan Jun Gan, Natalie A. Mellett, Partha P. Das, Jacquelyn M. Weir, Michael Ditiatkovski, Stacey Fynch, Peter Thorn, Helen E. Thomas, Peter J. Meikle, Helena C Parkington, Ian M. Smyth, Dmitri Sviridov

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Dysregulation of lipid homeostasis is intimately associated with defects in insulin secretion, a key feature of type 2 diabetes. Here, we explore the role of the putative lipid transporter ABCA12 in regulating insulin secretion from β-cells. Mice with β-cell-specific deletion of Abca12 display impaired glucose-stimulated insulin secretion and eventual islet inflammation and β-cell death. ABCA12's action in the pancreas is independent of changes in the abundance of two other cholesterol transporters, ABCA1 and ABCG1, or of changes in cellular cholesterol or ceramide content. Instead, loss of ABCA12 results in defects in the genesis and fusion of insulin secretory granules and increases in the abundance of lipid rafts at the cell membrane. These changes are associated with dysregulation of the small GTPase CDC42 and with decreased actin polymerisation. Our findings establish a new, pleiotropic role for ABCA12 in regulating pancreatic lipid homeostasis and insulin secretion.

Original languageEnglish
Article numbere48692
Number of pages19
JournalEMBO Reports
Volume21
Issue number3
DOIs
Publication statusPublished - 4 Mar 2020

Keywords

  • ABCA12
  • cholesterol homeostasis
  • insulin secretion
  • lipid rafts
  • type 2 diabetes

Cite this

Ursino, G. M., Fu, Y., Cottle, D. L., Mukhamedova, N., Jones, L. K., Low, H., Tham, M. S., Gan, W. J., Mellett, N. A., Das, P. P., Weir, J. M., Ditiatkovski, M., Fynch, S., Thorn, P., Thomas, H. E., Meikle, P. J., Parkington, H. C., Smyth, I. M., & Sviridov, D. (2020). ABCA12 regulates insulin secretion from β-cells. EMBO Reports, 21(3), [e48692]. https://doi.org/10.15252/embr.201948692