Ab initio studies of 2,4-diamino triazine and its complexes with ligands: A model for inhibitor-active site interactions of dihydrofolate reductase

Anne Marie Sapse, Mark C. Waltham, Joseph R. Bertino

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The protonation energies of 2,4-diamino triazine, an inhibitor of the therapeutic target dihydrofolate reductase, has been calculated using ab initio (Hartree-Fock) calculations. It is found that N1 (see Fig. 1) exhibits the highest proton affinity (261.6 kcal/mol) by comparison with other inhibitor protonation sites. The energies of binding of the formate ion and formamide (as models for the amino acid residues in the active site of dihydrofolate reductase) to neutral and protonated 2,4-diamino triazine are also obtained. The highest binding energies are featured by the complex formed from a formate attached to the N4 and N1 protonated forms of the triazine. However, as N4 has a comparatively low proton affinity (195.0 kcal/mol), it is unlikely that an interaction of this nature would prevail. On the other hand, the formate-protonated N1 interaction is similar to the structures identified by X-ray crystallography of enzyme-triazine complexes.

Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalCancer Investigation
Issue number5
Publication statusPublished - 1 Jan 1994
Externally publishedYes

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