AAV vectors encoding malarial antigens stimulate antigen-specific immunity but do not protect from parasite infection

Grant J Logan, Lina Wang, Maolin Zheng, Sharon C Cunningham, Ross Leon Coppel, Ian E Alexander

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

This study explores the utility of recombinant adeno-associated virus (rAAV) as a genetic vaccine delivery system using muscle as a target tissue. A single injection of rAAV encoding the malarial antigens MSP4 (Plasmodium falciparum) or MSP4/5 (Plasmodium yoelii) stimulated long-term antigen-specific antibody responses. Anti-MSP4/5 immunity stimulated by AAV was not protective against P. yoelii infection and efforts taken to augment antibody responses against MSP4/5, either by priming with plasmid DNA or AAV and boosting with rAAV were unsuccessful. Alternative strategies such as inclusion of genetic adjuvants into the AAV vector will be necessary to stimulate an adequate level of anti-malarial protective immunity in this model. Crown Copyright (c) 2006 Published by Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1014 - 1022
Number of pages8
JournalVaccine
Volume25
Publication statusPublished - 2007

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