A yeast telomerase complex containing the Est1 recruitment protein is assembled early in the cell cycle

Timothy M. Tucey, Victoria Lundblad

Research output: Contribution to journalArticleOtherpeer-review

11 Citations (Scopus)

Abstract

In budding yeast, association of the Est1 regulatory protein with telomerase is thought to be limited to the late S phase, when telomere elongation occurs. By monitoring the stoichiometry of telomerase subunits, we show instead that a telomerase complex containing Est1 is assembled much earlier in the cell cycle. We also report a biochemical interaction between Est1 and the telomere binding protein Cdc13 that recapitulates the previously observed genetic relationship between EST1 and CDC13. This supports a model in which regulated binding of Cdc13 to chromosome termini dictates subsequent interaction of a recruitment-competent telomerase complex with telomeres.

Original languageEnglish
Pages (from-to)1131-1133
Number of pages3
JournalBiochemistry
Volume52
Issue number7
DOIs
Publication statusPublished - 19 Feb 2013
Externally publishedYes

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