TY - JOUR
T1 - A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells
AU - Nefzger, Christian M
AU - Jarde, Thierry
AU - Rossello, Fernando Jaime
AU - Horvay, Katja
AU - Knaupp, Anja S
AU - Powell, David R
AU - Chen, Joseph Yuanfeng
AU - Abud, Helen E
AU - Polo, Jose M
PY - 2016
Y1 - 2016
N2 - The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency.
AB - The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency.
UR - http://www.ncbi.nlm.nih.gov/pubmed/26923822
U2 - 10.1016/j.stemcr.2016.01.014
DO - 10.1016/j.stemcr.2016.01.014
M3 - Article
VL - 6
SP - 321
EP - 329
JO - Stem Cell Reports
JF - Stem Cell Reports
SN - 2213-6711
IS - 3
ER -