A versatile monoclonal antibody specific to human SERPINB5

Sonia SY Teoh; Hong Wang; Gail P Risbridger; James C Whisstock; Phillip I Bird

doi:10.1089/hyb.2012.0035

A versatile monoclonal antibody specific to human SERPINB5

Sonia SY Teoh, Hong Wang, Gail P Risbridger, James C Whisstock, Phillip I Bird

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Abstract

Maspin (SERPINB5) is a member of the Clade B subgroup of the large superfamily of serine protease inhibitors. It is proposed that maspin is a tumor suppressor; however, its molecular role remains to be elucidated. Here we report the characterization of a mouse monoclonal antibody directed against human maspin. This antibody, 16F7, recognizes maspin in both its native and denatured form, unlike several other commercial antibodies tested in this study. It will be a useful and versatile tool for future analyses of the biological function of maspin.

Original language	English
Pages (from-to)	333 - 339
Number of pages	7
Journal	Hybridoma
Volume	31
Issue number	5
DOIs	https://doi.org/10.1089/hyb.2012.0035
Publication status	Published - 2012

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10.1089/hyb.2012.0035

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abstract = "Maspin (SERPINB5) is a member of the Clade B subgroup of the large superfamily of serine protease inhibitors. It is proposed that maspin is a tumor suppressor; however, its molecular role remains to be elucidated. Here we report the characterization of a mouse monoclonal antibody directed against human maspin. This antibody, 16F7, recognizes maspin in both its native and denatured form, unlike several other commercial antibodies tested in this study. It will be a useful and versatile tool for future analyses of the biological function of maspin.",

author = "Teoh, {Sonia SY} and Hong Wang and Risbridger, {Gail P} and Whisstock, {James C} and Bird, {Phillip I}",

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T1 - A versatile monoclonal antibody specific to human SERPINB5

AU - Teoh, Sonia SY

AU - Wang, Hong

AU - Risbridger, Gail P

AU - Whisstock, James C

AU - Bird, Phillip I

PY - 2012

Y1 - 2012

N2 - Maspin (SERPINB5) is a member of the Clade B subgroup of the large superfamily of serine protease inhibitors. It is proposed that maspin is a tumor suppressor; however, its molecular role remains to be elucidated. Here we report the characterization of a mouse monoclonal antibody directed against human maspin. This antibody, 16F7, recognizes maspin in both its native and denatured form, unlike several other commercial antibodies tested in this study. It will be a useful and versatile tool for future analyses of the biological function of maspin.

AB - Maspin (SERPINB5) is a member of the Clade B subgroup of the large superfamily of serine protease inhibitors. It is proposed that maspin is a tumor suppressor; however, its molecular role remains to be elucidated. Here we report the characterization of a mouse monoclonal antibody directed against human maspin. This antibody, 16F7, recognizes maspin in both its native and denatured form, unlike several other commercial antibodies tested in this study. It will be a useful and versatile tool for future analyses of the biological function of maspin.

UR - http://online.liebertpub.com/doi/pdf/10.1089/hyb.2012.0035

U2 - 10.1089/hyb.2012.0035

DO - 10.1089/hyb.2012.0035

M3 - Article

VL - 31

SP - 333

EP - 339

JO - Hybridoma and Hybridomics

JF - Hybridoma and Hybridomics

SN - 1554-0014

IS - 5

ER -

A versatile monoclonal antibody specific to human SERPINB5

Abstract

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