A vector, pSHT, for the expression and secretion of protein domains in mammalian cells

Edwin L. Madison, Phillip Bird

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18 Citations (Scopus)

Abstract

A phagemid (pSHT) containing the pUC and M13 ori sequences was constructed to facilitate the expression of partial cDNAs or of sequences encoding mammalian membrane- and secretory-protein domains. It provides a start codon and signal sequence flanked upstream by the simian virus 40 and bacteriophage T7 promoters and downstream by cloning sites, stop codons in all three frames, splicing and polyadenylation signals.

Original languageEnglish
Pages (from-to)179-180
Number of pages2
JournalGene
Volume121
Issue number1
DOIs
Publication statusPublished - 2 Nov 1992
Externally publishedYes

Keywords

  • expression vector
  • phagemid
  • protein secretion
  • Recombinant DNA

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