A vector, pSHT, for the expression and secretion of protein domains in mammalian cells

Edwin L. Madison; Phillip Bird

doi:10.1016/0378-1119(92)90179-S

A vector, pSHT, for the expression and secretion of protein domains in mammalian cells

Edwin L. Madison, Phillip Bird

Research output: Contribution to journal › Article › Research › peer-review

21 Citations (Scopus)

Abstract

A phagemid (pSHT) containing the pUC and M13 ori sequences was constructed to facilitate the expression of partial cDNAs or of sequences encoding mammalian membrane- and secretory-protein domains. It provides a start codon and signal sequence flanked upstream by the simian virus 40 and bacteriophage T7 promoters and downstream by cloning sites, stop codons in all three frames, splicing and polyadenylation signals.

Original language	English
Pages (from-to)	179-180
Number of pages	2
Journal	Gene
Volume	121
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(92)90179-S
Publication status	Published - 2 Nov 1992
Externally published	Yes

Keywords

expression vector
phagemid
protein secretion
Recombinant DNA

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10.1016/0378-1119(92)90179-S

Cite this

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abstract = "A phagemid (pSHT) containing the pUC and M13 ori sequences was constructed to facilitate the expression of partial cDNAs or of sequences encoding mammalian membrane- and secretory-protein domains. It provides a start codon and signal sequence flanked upstream by the simian virus 40 and bacteriophage T7 promoters and downstream by cloning sites, stop codons in all three frames, splicing and polyadenylation signals.",

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AU - Bird, Phillip

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N2 - A phagemid (pSHT) containing the pUC and M13 ori sequences was constructed to facilitate the expression of partial cDNAs or of sequences encoding mammalian membrane- and secretory-protein domains. It provides a start codon and signal sequence flanked upstream by the simian virus 40 and bacteriophage T7 promoters and downstream by cloning sites, stop codons in all three frames, splicing and polyadenylation signals.

AB - A phagemid (pSHT) containing the pUC and M13 ori sequences was constructed to facilitate the expression of partial cDNAs or of sequences encoding mammalian membrane- and secretory-protein domains. It provides a start codon and signal sequence flanked upstream by the simian virus 40 and bacteriophage T7 promoters and downstream by cloning sites, stop codons in all three frames, splicing and polyadenylation signals.

KW - expression vector

KW - phagemid

KW - protein secretion

KW - Recombinant DNA

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U2 - 10.1016/0378-1119(92)90179-S

DO - 10.1016/0378-1119(92)90179-S

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AN - SCOPUS:0026442805

SN - 0378-1119

VL - 121

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EP - 180

JO - Gene

JF - Gene

IS - 1

ER -

A vector, pSHT, for the expression and secretion of protein domains in mammalian cells

Abstract

Keywords

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