A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes

Makoto Furutani-Seiki, Takao Sasado, Chikako Morinaga, Hiroshi Suwa, Katsutoshi Niwa, Hiroki Yoda, Tomonori Deguchi, Yukihiro Hirose, Akihito Yasuoka, Thorsten Henrich, Tomomi Watanabe, Norimasa Iwanami, Daiju Kitagawa, Kota Saito, Satoshi Asaka, Masakazu Osakada, Sanae Kunimatsu, Akihiro Momoi, Harun Elmasri, Christoph WinklerMirana Ramialison, Felix Loosli, Rebecca Quiring, Matthias Carl, Clemens Grabher, Sylke Winkler, Filippo Del Bene, Ai Shinomiya, Yasuko Kota, Toshiyuki Yamanaka, Yasuko Okamoto, Katsuhito Takahashi, Takeshi Todo, Keiko Abe, Yousuke Takahama, Minoru Tanaka, Hiroshi Mitani, Toshiaki Katada, Hiroshi Nishina, Noboru Nakajima, Joachim Wittbrodt, Hisato Kondoh

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95 Citations (Scopus)

Abstract

A large-scale mutagenesis screen was performed in Medaka to identify genes acting in diverse developmental processes. Mutations were identified in homozygous F3 progeny derived from ENU-treated founder males. In addition to the morphological inspection of live embryos, other approaches were used to detect abnormalities in organogenesis and in specific cellular processes, including germ cell migration, nerve tract formation, sensory organ differentiation and DNA repair. Among 2031 embryonic lethal mutations identified, 312 causing defects in organogenesis were selected for further analyses. From these, 126 mutations were characterized genetically and assigned to 105 genes. The similarity of the development of Medaka and zebrafish facilitated the comparison of mutant phenotypes, which indicated that many mutations in Medaka cause unique phenotypes so far unrecorded in zebrafish. Even when mutations of the two fish species cause a similar phenotype such as one-eyed-pinhead or parachute, more genes were found in Medaka than in zebrafish that produced the same phenotype when mutated. These observations suggest that many Medaka mutants represent new genes and, therefore, are important complements to the collection of zebrafish mutants that have proven so valuable for exploring genomic function in development. 

Original languageEnglish
Pages (from-to)647-658
Number of pages12
JournalMechanisms of Development
Volume121
Issue number7-8
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

Keywords

  • ENU mutagenesis
  • Interspecies difference
  • Kyoto screen
  • Medaka
  • Mutants
  • Vertebrate organogenesis

Cite this

Furutani-Seiki, M., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Yoda, H., ... Kondoh, H. (2004). A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes. Mechanisms of Development, 121(7-8), 647-658. https://doi.org/10.1016/j.mod.2004.04.016