A synthetic peptide containing the autophosphorylation site of the transforming protein of Harvey sarcoma virus is phosphorylated by the EGF-stimulated tyrosine kinase

G. S. Baldwin; I. J. Stanley; E. C. Nice

doi:10.1016/0014-5793(83)80619-X

A synthetic peptide containing the autophosphorylation site of the transforming protein of Harvey sarcoma virus is phosphorylated by the EGF-stimulated tyrosine kinase

G. S. Baldwin, I. J. Stanley, E. C. Nice

Research output: Contribution to journal › Article › Research › peer-review

14 Citations (Scopus)

Abstract

The transforming proteins (p21) of Harvey and Kirsten sarcoma viruses threonine kinase activity, which phosphorylates threonine 59 of the p21 proteins themselves. A tridecapeptide: Arg-Arg-Leu⁵⁶-Asp-Thr-Thr⁵⁹-Gly-Gln-Glu-Tyr-Ser-Ala⁶⁶ containing residues 56-66 of p21 is phosphorylated solely on tyrosine by the epidermal growth factor (EGF)-stimulated tyrosine kinase of A431 cell membranes. K_m-Values of 240 and 80 μM and V_max values of 1.7 and 0.1 nmol.min^-1.mg^-1 were obtained in the presence and absence of EGF, respectively.

Original language	English
Pages (from-to)	257-261
Number of pages	5
Journal	FEBS Letters
Volume	153
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(83)80619-X
Publication status	Published - 21 Mar 1983
Externally published	Yes

Keywords

Epidermal growth factor receptor
Harvey virus oncogene
Kirsten virus oncogene
Phosphothreonine
Phosphotyrosine
Protein p21

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10.1016/0014-5793(83)80619-X

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title = "A synthetic peptide containing the autophosphorylation site of the transforming protein of Harvey sarcoma virus is phosphorylated by the EGF-stimulated tyrosine kinase",

abstract = "The transforming proteins (p21) of Harvey and Kirsten sarcoma viruses threonine kinase activity, which phosphorylates threonine 59 of the p21 proteins themselves. A tridecapeptide: Arg-Arg-Leu56-Asp-Thr-Thr59-Gly-Gln-Glu-Tyr-Ser-Ala66 containing residues 56-66 of p21 is phosphorylated solely on tyrosine by the epidermal growth factor (EGF)-stimulated tyrosine kinase of A431 cell membranes. Km-Values of 240 and 80 μM and Vmax values of 1.7 and 0.1 nmol.min-1.mg-1 were obtained in the presence and absence of EGF, respectively.",

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T1 - A synthetic peptide containing the autophosphorylation site of the transforming protein of Harvey sarcoma virus is phosphorylated by the EGF-stimulated tyrosine kinase

AU - Baldwin, G. S.

AU - Stanley, I. J.

AU - Nice, E. C.

PY - 1983/3/21

Y1 - 1983/3/21

N2 - The transforming proteins (p21) of Harvey and Kirsten sarcoma viruses threonine kinase activity, which phosphorylates threonine 59 of the p21 proteins themselves. A tridecapeptide: Arg-Arg-Leu56-Asp-Thr-Thr59-Gly-Gln-Glu-Tyr-Ser-Ala66 containing residues 56-66 of p21 is phosphorylated solely on tyrosine by the epidermal growth factor (EGF)-stimulated tyrosine kinase of A431 cell membranes. Km-Values of 240 and 80 μM and Vmax values of 1.7 and 0.1 nmol.min-1.mg-1 were obtained in the presence and absence of EGF, respectively.

AB - The transforming proteins (p21) of Harvey and Kirsten sarcoma viruses threonine kinase activity, which phosphorylates threonine 59 of the p21 proteins themselves. A tridecapeptide: Arg-Arg-Leu56-Asp-Thr-Thr59-Gly-Gln-Glu-Tyr-Ser-Ala66 containing residues 56-66 of p21 is phosphorylated solely on tyrosine by the epidermal growth factor (EGF)-stimulated tyrosine kinase of A431 cell membranes. Km-Values of 240 and 80 μM and Vmax values of 1.7 and 0.1 nmol.min-1.mg-1 were obtained in the presence and absence of EGF, respectively.

KW - Epidermal growth factor receptor

KW - Harvey virus oncogene

KW - Kirsten virus oncogene

KW - Phosphothreonine

KW - Phosphotyrosine

KW - Protein p21

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DO - 10.1016/0014-5793(83)80619-X

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AN - SCOPUS:0020575182

VL - 153

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JO - FEBS Letters

JF - FEBS Letters

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ER -

A synthetic peptide containing the autophosphorylation site of the transforming protein of Harvey sarcoma virus is phosphorylated by the EGF-stimulated tyrosine kinase

Abstract

Keywords

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