A superovulation protocol for the spiny mouse (Acomys cahirinus)

Rachael Pasco, David K Gardner, David William Walker, Hayley Dickinson

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

This study aimed to develop a superovulation protocol for the spiny mouse (Acomys cahirinus). The spiny mouse is a desert-adapted rodent species, with a long oestrus cycle (11 days) compared with rat and mouse, and gives birth to few (mean litter size is 3) precocial offspring after a relatively long gestation (39 days). We successfully optimised a superovulation protocol that elicited a 5-fold increase in the normal ovulation rate of this species. To induce superovulation in the spiny mouse 2 injections of equine chorionic gonadotrophin (eCG, 10IU each), 9 h apart, were required, followed by 20 IU of human chorionic gonadotrophin (hCG). This protocol was successful in 100 of females trialed and at 33 h post-hCG an average of 14.7 ?1.5,1-2 cell embryos were recovered. Histological analysis of ovaries following superovulation revealed large corpus lutea and post-ovulatory follicles occupying a large part of the ovary. Ovulation commenced 6-12 h after the hCG injection and continued until 24-33 h post-hCG as indicated by both histological analysis of ovaries and the presence of oocytes/embryos in the oviduct. This superovulation protocol will facilitate the development of an in vitro culture system for spiny mouse embryos.
Original languageEnglish
Pages (from-to)1117 - 1122
Number of pages6
JournalReproduction, Fertility and Development
Volume24
Issue number8
DOIs
Publication statusPublished - 2012

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